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Effect of lipase on monoolein-based cubic phase dispersion (cubosomes) and vesicles

Borné, Johanna LU ; Nylander, Tommy LU and Khan, Ali LU (2002) In The Journal of Physical Chemistry Part B 106(40). p.10492-10500
Abstract
The effect of adding a Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a cubic dispersion of the binary monoolein (MO)-(H2O)-H-2 system and to vesicles formed by the ternary MO-sodium oleate (NaO)(H2O)-H-2 system at high water content has been studied. The change in lipid aggregate structure during the lipolytic process was observed by light microscopy, cryo-TEM, and dynamic light scattering, and the lipid composition was determined with HPLC. The sequence of lipid aggregate structures observed upon lipase addition to the cubic dispersion is cubic dispersion --> reversed cubic, hexagonal, and lamellar structures --> large polygonal structures --> dispersed oil droplets. The addition of lipase to MO/NaO aqueous-based... (More)
The effect of adding a Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a cubic dispersion of the binary monoolein (MO)-(H2O)-H-2 system and to vesicles formed by the ternary MO-sodium oleate (NaO)(H2O)-H-2 system at high water content has been studied. The change in lipid aggregate structure during the lipolytic process was observed by light microscopy, cryo-TEM, and dynamic light scattering, and the lipid composition was determined with HPLC. The sequence of lipid aggregate structures observed upon lipase addition to the cubic dispersion is cubic dispersion --> reversed cubic, hexagonal, and lamellar structures --> large polygonal structures --> dispersed oil droplets. The addition of lipase to MO/NaO aqueous-based vesicles did not change the morphology significantly. However, close to steady state, the vesicles changed from having smooth interfaces to irregular ones. When lipolysis reached steady state, the MO/(OA+NaO) ratio was similar in the two systems studied, although the morphology of the structures formed during lipolysis differed significantly. These differences are discussed in relation to the degree of dissociation of the fatty acid. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
106
issue
40
pages
10492 - 10500
publisher
The American Chemical Society
external identifiers
  • wos:000178506900030
  • scopus:0037058097
ISSN
1520-5207
DOI
10.1021/jp021023y
language
English
LU publication?
yes
id
81523ec9-2a0b-4c5e-84ab-348ee9c5e968 (old id 326095)
date added to LUP
2007-08-10 14:38:26
date last changed
2017-07-23 04:37:25
@article{81523ec9-2a0b-4c5e-84ab-348ee9c5e968,
  abstract     = {The effect of adding a Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a cubic dispersion of the binary monoolein (MO)-(H2O)-H-2 system and to vesicles formed by the ternary MO-sodium oleate (NaO)(H2O)-H-2 system at high water content has been studied. The change in lipid aggregate structure during the lipolytic process was observed by light microscopy, cryo-TEM, and dynamic light scattering, and the lipid composition was determined with HPLC. The sequence of lipid aggregate structures observed upon lipase addition to the cubic dispersion is cubic dispersion --> reversed cubic, hexagonal, and lamellar structures --> large polygonal structures --> dispersed oil droplets. The addition of lipase to MO/NaO aqueous-based vesicles did not change the morphology significantly. However, close to steady state, the vesicles changed from having smooth interfaces to irregular ones. When lipolysis reached steady state, the MO/(OA+NaO) ratio was similar in the two systems studied, although the morphology of the structures formed during lipolysis differed significantly. These differences are discussed in relation to the degree of dissociation of the fatty acid.},
  author       = {Borné, Johanna and Nylander, Tommy and Khan, Ali},
  issn         = {1520-5207},
  language     = {eng},
  number       = {40},
  pages        = {10492--10500},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Effect of lipase on monoolein-based cubic phase dispersion (cubosomes) and vesicles},
  url          = {http://dx.doi.org/10.1021/jp021023y},
  volume       = {106},
  year         = {2002},
}