Selective breeding of mice for different susceptibilities to high fat diet-induced glucose intolerance : Development of two novel mouse lines, Selectively bred Diet-induced Glucose intolerance-Prone and -Resistant
(2012) In Journal of Diabetes Investigation 3(3). p.245-251- Abstract
Aims/Introduction: The development of type 2 diabetes is primarily due to lifestyle and environmental factors, as well as genetics, as shown by familial clustering. To establish mouse lines for evaluating heritable factors determining susceptibility to diet-induced diabetes, we performed selective breeding for differences in high fat diet (HFD)-induced glucose intolerance. Materials and Methods: Selective breeding was performed using hybrid mice of C57BL/6J, C3H/HeJ and AKR/N backgrounds. After 5-week HFD feeding, mice showing high and low 2-h blood glucose levels in an oral glucose tolerance test (OGTT) were selected and bred over 14 generations to produce lines prone and resistant to diet-induced glucose intolerance (designated... (More)
Aims/Introduction: The development of type 2 diabetes is primarily due to lifestyle and environmental factors, as well as genetics, as shown by familial clustering. To establish mouse lines for evaluating heritable factors determining susceptibility to diet-induced diabetes, we performed selective breeding for differences in high fat diet (HFD)-induced glucose intolerance. Materials and Methods: Selective breeding was performed using hybrid mice of C57BL/6J, C3H/HeJ and AKR/N backgrounds. After 5-week HFD feeding, mice showing high and low 2-h blood glucose levels in an oral glucose tolerance test (OGTT) were selected and bred over 14 generations to produce lines prone and resistant to diet-induced glucose intolerance (designated Selectively bred Diet-induced Glucose intolerance-Prone [SDG-P] and -Resistant [SDG-R]). Results: At 5weeks of age (pre HFD feeding), SDG-P mice showed higher blood glucose levels both in the OGTT and insulin tolerance test as compared to SDG-R mice. After receiving HFD, the glucose intolerance of SDG-P mice became more evident without hyper insulin secretion. In addition, SDG-P mice had greater body weight gain and lower HDL-cholesterol levels as compared to SDG-R mice. In comparison with C57BL/6J, a well-known strain prone to HFD-induced glucose intolerance, SDG-P mice showed significantly higher glucose levels in OGTT after the 5-week HFD feeding. Conclusions: Susceptibility to HFD-induced glucose intolerance was transmitted over generations and was intensified by selective breeding. The newly established mouse lines, SDG-P and SDG-R, may be useful in investigating the pathophysiology of type 2 diabetes through elucidating the crucial factors for determining the susceptibility to HFD-induced glucose intolerance.
(Less)
- author
- publishing date
- 2012-06
- type
- Contribution to journal
- publication status
- published
- keywords
- Dietary fats, Glucose intolerance, Mice
- in
- Journal of Diabetes Investigation
- volume
- 3
- issue
- 3
- pages
- 245 - 251
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:84861983614
- ISSN
- 2040-1116
- DOI
- 10.1111/j.2040-1124.2011.00175.x
- language
- English
- LU publication?
- no
- id
- 3261fba3-08e2-4a0e-b249-21cabbfad0f2
- date added to LUP
- 2017-08-23 20:04:43
- date last changed
- 2022-01-30 22:14:55
@article{3261fba3-08e2-4a0e-b249-21cabbfad0f2,
abstract = {{<p>Aims/Introduction: The development of type 2 diabetes is primarily due to lifestyle and environmental factors, as well as genetics, as shown by familial clustering. To establish mouse lines for evaluating heritable factors determining susceptibility to diet-induced diabetes, we performed selective breeding for differences in high fat diet (HFD)-induced glucose intolerance. Materials and Methods: Selective breeding was performed using hybrid mice of C57BL/6J, C3H/HeJ and AKR/N backgrounds. After 5-week HFD feeding, mice showing high and low 2-h blood glucose levels in an oral glucose tolerance test (OGTT) were selected and bred over 14 generations to produce lines prone and resistant to diet-induced glucose intolerance (designated Selectively bred Diet-induced Glucose intolerance-Prone [SDG-P] and -Resistant [SDG-R]). Results: At 5weeks of age (pre HFD feeding), SDG-P mice showed higher blood glucose levels both in the OGTT and insulin tolerance test as compared to SDG-R mice. After receiving HFD, the glucose intolerance of SDG-P mice became more evident without hyper insulin secretion. In addition, SDG-P mice had greater body weight gain and lower HDL-cholesterol levels as compared to SDG-R mice. In comparison with C57BL/6J, a well-known strain prone to HFD-induced glucose intolerance, SDG-P mice showed significantly higher glucose levels in OGTT after the 5-week HFD feeding. Conclusions: Susceptibility to HFD-induced glucose intolerance was transmitted over generations and was intensified by selective breeding. The newly established mouse lines, SDG-P and SDG-R, may be useful in investigating the pathophysiology of type 2 diabetes through elucidating the crucial factors for determining the susceptibility to HFD-induced glucose intolerance.</p>}},
author = {{Nagao, Mototsugu and Asai, Akira and Kawahara, Momoyo and Nakajima, Yasushi and Sato, Yuki and Tanimura-Inagaki, Kyoko and Okajima, Fumitaka and Takaya, Makiyo and Sudo, Mariko and Takemitsu, Shuji and Harada, Taro and Sugihara, Hitoshi and Oikawa, Shinichi}},
issn = {{2040-1116}},
keywords = {{Dietary fats; Glucose intolerance; Mice}},
language = {{eng}},
number = {{3}},
pages = {{245--251}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Diabetes Investigation}},
title = {{Selective breeding of mice for different susceptibilities to high fat diet-induced glucose intolerance : Development of two novel mouse lines, Selectively bred Diet-induced Glucose intolerance-Prone and -Resistant}},
url = {{http://dx.doi.org/10.1111/j.2040-1124.2011.00175.x}},
doi = {{10.1111/j.2040-1124.2011.00175.x}},
volume = {{3}},
year = {{2012}},
}