Advanced

Induction of differentiation in acute promyelocytic leukemia cells (HL-60) by the verticillin derivative Sch 52900

Erkel, G; Gehrt, A; Anke, T and Sterner, Olov LU (2002) In Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences 57(7-8). p.759-767
Abstract
The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. In a screening for inducers of differentiation of HL-60 cells, cultures of Gliocladium strain 4-93 were found to produce Sch 52900, a previously isolated diketopiperazine (Chu et al,. J. Antibiotics 48, 1440-1445). Sch 52900 induced the differentation of 50-69% of HL-60 cells at concentrations of 6.8-13.6 nm as measured by nitro-blue tetrazolium chloride (NBT) reduction which was followed by apoptosis as shown by DNA fragmentation. Our results demonstrate that growth arrest and the induction of differentiation by Sch 52900 is due to the... (More)
The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. In a screening for inducers of differentiation of HL-60 cells, cultures of Gliocladium strain 4-93 were found to produce Sch 52900, a previously isolated diketopiperazine (Chu et al,. J. Antibiotics 48, 1440-1445). Sch 52900 induced the differentation of 50-69% of HL-60 cells at concentrations of 6.8-13.6 nm as measured by nitro-blue tetrazolium chloride (NBT) reduction which was followed by apoptosis as shown by DNA fragmentation. Our results demonstrate that growth arrest and the induction of differentiation by Sch 52900 is due to the induction of the cell cycle inhibitor p21(WAF) and an inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway which leads to the activation of the transcription factor AP-1. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
differentiation induction, AP-1, p21(WAF)
in
Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences
volume
57
issue
7-8
pages
759 - 767
publisher
Verlag der Zeitschrift für Naturforschung
external identifiers
  • wos:000178355500034
  • scopus:0036630871
ISSN
0939-5075
language
English
LU publication?
yes
id
3e126d9a-e1b1-40c7-9b5d-aee4b6cc122e (old id 326269)
alternative location
http://www.znaturforsch.com/ac/v57c/s57c0759.pdf
date added to LUP
2007-08-17 11:01:48
date last changed
2017-01-01 07:28:24
@article{3e126d9a-e1b1-40c7-9b5d-aee4b6cc122e,
  abstract     = {The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. In a screening for inducers of differentiation of HL-60 cells, cultures of Gliocladium strain 4-93 were found to produce Sch 52900, a previously isolated diketopiperazine (Chu et al,. J. Antibiotics 48, 1440-1445). Sch 52900 induced the differentation of 50-69% of HL-60 cells at concentrations of 6.8-13.6 nm as measured by nitro-blue tetrazolium chloride (NBT) reduction which was followed by apoptosis as shown by DNA fragmentation. Our results demonstrate that growth arrest and the induction of differentiation by Sch 52900 is due to the induction of the cell cycle inhibitor p21(WAF) and an inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway which leads to the activation of the transcription factor AP-1.},
  author       = {Erkel, G and Gehrt, A and Anke, T and Sterner, Olov},
  issn         = {0939-5075},
  keyword      = {differentiation induction,AP-1,p21(WAF)},
  language     = {eng},
  number       = {7-8},
  pages        = {759--767},
  publisher    = {Verlag der Zeitschrift für Naturforschung},
  series       = {Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences},
  title        = {Induction of differentiation in acute promyelocytic leukemia cells (HL-60) by the verticillin derivative Sch 52900},
  volume       = {57},
  year         = {2002},
}