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Establishment and characterisation of a human clear cell sarcoma model in nude mice

Crnalic, S; Panagopoulos, I; Boquist, L; Mandahl, Nils LU ; Stenling, R and Lofvenberg, R (2002) In International Journal of Cancer 101(6). p.505-511
Abstract
We have established a new experimental model of human clear cell sarcoma, UM-CCSI, using serial subcutaneous transplantation of intact tumour tissue in nude mice. The heterotransplanted nude mouse tumours retained characteristic morphological features of the primary clear cell sarcoma. Immunohistochemical analysis showed the retained expression patterns of S-100 protein, melanoma-associated antigen HMB-45 and vimentin in the xenografts as compared to the primary tumour. DNA index showed low variations both between the xenografts in the same passage and between the serial passages. Cytogenetic analysis of the primary tumour and the xenografts showed the unbalanced translocation der(6)t(6; I 2)(p23;q13). Based on the combined genetic data a... (More)
We have established a new experimental model of human clear cell sarcoma, UM-CCSI, using serial subcutaneous transplantation of intact tumour tissue in nude mice. The heterotransplanted nude mouse tumours retained characteristic morphological features of the primary clear cell sarcoma. Immunohistochemical analysis showed the retained expression patterns of S-100 protein, melanoma-associated antigen HMB-45 and vimentin in the xenografts as compared to the primary tumour. DNA index showed low variations both between the xenografts in the same passage and between the serial passages. Cytogenetic analysis of the primary tumour and the xenografts showed the unbalanced translocation der(6)t(6; I 2)(p23;q13). Based on the combined genetic data a reasonable interpretation of our findings is that there was a complex chromosomal rearrangement resulting in a cytogenetically cryptic EWS-ATFI fusion gene. Analysis of cell kinetics using in vivo incorporation of iododeoxyuridine and flow cytometry showed generally short potential doubling time (T-pot) of the xenografts. Volume doubling time showed low variations without correlation with T-pot. The retained phenotypic and genotypic characteristics of the primary tumour and the morphological and structural stability over time makes the model suitable for studies on the tumour biology and treatment of clear cell sarcoma. (C) 2002 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunohistochemistry, cytogenetics, clear cell sarcoma, flow cytometry, EWS/ATF1 transcript, nude mice
in
International Journal of Cancer
volume
101
issue
6
pages
505 - 511
publisher
John Wiley & Sons
external identifiers
  • wos:000178250900001
  • pmid:12237889
  • scopus:0037145327
ISSN
0020-7136
DOI
10.1002/ijc.10588
language
English
LU publication?
yes
id
e118168c-d069-40fa-b895-107ba7983fc4 (old id 326577)
date added to LUP
2007-08-15 12:13:38
date last changed
2017-01-01 04:46:14
@article{e118168c-d069-40fa-b895-107ba7983fc4,
  abstract     = {We have established a new experimental model of human clear cell sarcoma, UM-CCSI, using serial subcutaneous transplantation of intact tumour tissue in nude mice. The heterotransplanted nude mouse tumours retained characteristic morphological features of the primary clear cell sarcoma. Immunohistochemical analysis showed the retained expression patterns of S-100 protein, melanoma-associated antigen HMB-45 and vimentin in the xenografts as compared to the primary tumour. DNA index showed low variations both between the xenografts in the same passage and between the serial passages. Cytogenetic analysis of the primary tumour and the xenografts showed the unbalanced translocation der(6)t(6; I 2)(p23;q13). Based on the combined genetic data a reasonable interpretation of our findings is that there was a complex chromosomal rearrangement resulting in a cytogenetically cryptic EWS-ATFI fusion gene. Analysis of cell kinetics using in vivo incorporation of iododeoxyuridine and flow cytometry showed generally short potential doubling time (T-pot) of the xenografts. Volume doubling time showed low variations without correlation with T-pot. The retained phenotypic and genotypic characteristics of the primary tumour and the morphological and structural stability over time makes the model suitable for studies on the tumour biology and treatment of clear cell sarcoma. (C) 2002 Wiley-Liss, Inc.},
  author       = {Crnalic, S and Panagopoulos, I and Boquist, L and Mandahl, Nils and Stenling, R and Lofvenberg, R},
  issn         = {0020-7136},
  keyword      = {immunohistochemistry,cytogenetics,clear cell sarcoma,flow cytometry,EWS/ATF1 transcript,nude mice},
  language     = {eng},
  number       = {6},
  pages        = {505--511},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Establishment and characterisation of a human clear cell sarcoma model in nude mice},
  url          = {http://dx.doi.org/10.1002/ijc.10588},
  volume       = {101},
  year         = {2002},
}