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Immunolocalization of cholecystokinin-2 receptors in rat gastric mucosa

Tommeras, K; Hammer, P; Sundler, Frank LU ; Borch, K; Mardh, S and Cabero, JL (2002) In Scandinavian Journal of Gastroenterology 37(9). p.1017-1024
Abstract
Background: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin. Methods: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats 1 h before they were killed, the acid pump H, K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase. Results: Undifferentiated foetal gastric epithelial cells... (More)
Background: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin. Methods: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats 1 h before they were killed, the acid pump H, K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase. Results: Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H, K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors. Conclusion: Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
organogenesis, immunofluorescence, CCK2 receptor, gastrin, progenitor, zone, stomach
in
Scandinavian Journal of Gastroenterology
volume
37
issue
9
pages
1017 - 1024
publisher
Taylor & Francis
external identifiers
  • pmid:12374225
  • wos:000178104100005
  • scopus:18644385596
ISSN
1502-7708
DOI
10.1080/003655202320378194
language
English
LU publication?
yes
id
4e5662ea-e0f6-4e2d-9d90-1007500bf3b7 (old id 327839)
date added to LUP
2007-11-16 17:36:04
date last changed
2017-01-01 07:14:10
@article{4e5662ea-e0f6-4e2d-9d90-1007500bf3b7,
  abstract     = {Background: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin. Methods: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats 1 h before they were killed, the acid pump H, K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase. Results: Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H, K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors. Conclusion: Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life.},
  author       = {Tommeras, K and Hammer, P and Sundler, Frank and Borch, K and Mardh, S and Cabero, JL},
  issn         = {1502-7708},
  keyword      = {organogenesis,immunofluorescence,CCK2 receptor,gastrin,progenitor,zone,stomach},
  language     = {eng},
  number       = {9},
  pages        = {1017--1024},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Immunolocalization of cholecystokinin-2 receptors in rat gastric mucosa},
  url          = {http://dx.doi.org/10.1080/003655202320378194},
  volume       = {37},
  year         = {2002},
}