A reevaluation of the validity of unrestrained plethysmography in mice
(2002) In Journal of Applied Physiology 93(4). p.1198-1207- Abstract
- Presently, unrestrained plethysmography is widely used to assess bronchial responsiveness in mice. An empirical quantity known as enhanced pause is derived from the plethysmographic box pressure [P-b( t), where t is time] and assumed to be an index of bronchoconstriction. We show that P-b(t) is determined largely by gas conditioning when normal mice breathe spontaneously inside a closed chamber in which the air is at ambient conditions. When the air in the chamber is heated and humidified to body conditions, the changes in P-b(t) are reduced by about two-thirds. The remaining changes are thus due to gas compression and expansion within the lung and are amplified when the animals breathe through increased resistances. We show that the time... (More)
- Presently, unrestrained plethysmography is widely used to assess bronchial responsiveness in mice. An empirical quantity known as enhanced pause is derived from the plethysmographic box pressure [P-b( t), where t is time] and assumed to be an index of bronchoconstriction. We show that P-b(t) is determined largely by gas conditioning when normal mice breathe spontaneously inside a closed chamber in which the air is at ambient conditions. When the air in the chamber is heated and humidified to body conditions, the changes in P-b(t) are reduced by about two-thirds. The remaining changes are thus due to gas compression and expansion within the lung and are amplified when the animals breathe through increased resistances. We show that the time integral of P-b(t) over inspiration is accurately predicted by a term containing airway resistance, functional residual capacity, and tidal volume. We conclude that unrestrained plethysmography can be used to accurately characterize changes in airway resistance only if functional residual capacity and tidal volume are measured independently and the chamber gas is preconditioned to body temperature and humidity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/328587
- author
- Lundblad, Lennart LU ; Irvin, CG ; Adler, A and Bates, JHT
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- airway resistance, Boyle's law, gas conditioning
- in
- Journal of Applied Physiology
- volume
- 93
- issue
- 4
- pages
- 1198 - 1207
- publisher
- American Physiological Society
- external identifiers
-
- wos:000178034600004
- pmid:12235015
- scopus:0036785044
- ISSN
- 1522-1601
- DOI
- 10.1152/japplphysiol.00080.2002
- language
- English
- LU publication?
- yes
- id
- a5ca24c3-47be-40fb-a09d-052ca80fa660 (old id 328587)
- alternative location
- http://jap.physiology.org/cgi/reprint/00080.2002v1
- date added to LUP
- 2016-04-01 12:05:27
- date last changed
- 2023-09-15 19:26:45
@article{a5ca24c3-47be-40fb-a09d-052ca80fa660,
abstract = {{Presently, unrestrained plethysmography is widely used to assess bronchial responsiveness in mice. An empirical quantity known as enhanced pause is derived from the plethysmographic box pressure [P-b( t), where t is time] and assumed to be an index of bronchoconstriction. We show that P-b(t) is determined largely by gas conditioning when normal mice breathe spontaneously inside a closed chamber in which the air is at ambient conditions. When the air in the chamber is heated and humidified to body conditions, the changes in P-b(t) are reduced by about two-thirds. The remaining changes are thus due to gas compression and expansion within the lung and are amplified when the animals breathe through increased resistances. We show that the time integral of P-b(t) over inspiration is accurately predicted by a term containing airway resistance, functional residual capacity, and tidal volume. We conclude that unrestrained plethysmography can be used to accurately characterize changes in airway resistance only if functional residual capacity and tidal volume are measured independently and the chamber gas is preconditioned to body temperature and humidity.}},
author = {{Lundblad, Lennart and Irvin, CG and Adler, A and Bates, JHT}},
issn = {{1522-1601}},
keywords = {{airway resistance; Boyle's law; gas conditioning}},
language = {{eng}},
number = {{4}},
pages = {{1198--1207}},
publisher = {{American Physiological Society}},
series = {{Journal of Applied Physiology}},
title = {{A reevaluation of the validity of unrestrained plethysmography in mice}},
url = {{http://dx.doi.org/10.1152/japplphysiol.00080.2002}},
doi = {{10.1152/japplphysiol.00080.2002}},
volume = {{93}},
year = {{2002}},
}