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Cerebral ischemia upregulates vascular endothelin ETB receptors in rat

Stenman, Emelie LU ; Malmsjö, Malin LU ; Uddman, Erik LU ; Gidö, Gunilla LU ; Wieloch, Tadeusz LU and Edvinsson, Lars LU (2002) In Stroke: a journal of cerebral circulation 33(9). p.2311-2316
Abstract
Background and Purpose-Elevated levels of endothelin 1 (ET 1) have been reported in cerebral ischemia A role for ET may prove more important if the vascular receptors were changed We addressed whether there is any change in ET receptor expression in cerebral ischemia Methods-The right middle cerebral artery (MCA) was occluded in male Wistar rats for 2 hours with the intraluminal filament method The basilar artery and both MCAs were removed after 46 hours of recirculation The contractile responses to ET 1 a combined ETA and ETB receptor agonist and sarafotoxin 6c (S6c) a selective ETB receptor agonist were examined in vitro and ET receptor mRNA was quantified by real time polymerase chain reaction Results-S6c which had no contractile effect... (More)
Background and Purpose-Elevated levels of endothelin 1 (ET 1) have been reported in cerebral ischemia A role for ET may prove more important if the vascular receptors were changed We addressed whether there is any change in ET receptor expression in cerebral ischemia Methods-The right middle cerebral artery (MCA) was occluded in male Wistar rats for 2 hours with the intraluminal filament method The basilar artery and both MCAs were removed after 46 hours of recirculation The contractile responses to ET 1 a combined ETA and ETB receptor agonist and sarafotoxin 6c (S6c) a selective ETB receptor agonist were examined in vitro and ET receptor mRNA was quantified by real time polymerase chain reaction Results-S6c which had no contractile effect per se on fresh or sham operated rat cerebral arteries induced a marked contraction in the occluded MCA (E-m [maximum contraction calculated as percentage of the contractile capacity of 63 5 mmol/L K+] =68+/-68% P<0 0001) while there was no difference in the responses to ET 1 after cerebral ischemia Real time polymerase chain reaction revealed a significant upregulation of both the ETA and ETB receptors (both P<0 05) in the occluded MCA compared with the nonoccluded MCA from the same rats Conclusions-Focal cerebral ischemia in rat induces increased transcription of both ETA and ETB receptors which results in the appearance of a contractile response to the ETB receptor agonist S6c These results suggest a role for ET receptors in the pathogenesis of a vascular component after cerebral ischemia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
receptors, endothelins, middle cerebral artery, cerebral ischemia, endothelin, rats
in
Stroke: a journal of cerebral circulation
volume
33
issue
9
pages
2311 - 2316
publisher
American Heart Association
external identifiers
  • pmid:12215604
  • wos:000177934400046
  • scopus:0036713458
ISSN
1524-4628
DOI
10.1161/01.STR.0000028183.04277.32
language
English
LU publication?
yes
id
9517f6fc-b471-4e03-be39-d3cf4c6061a1 (old id 328672)
date added to LUP
2007-11-14 14:25:29
date last changed
2017-10-01 04:51:51
@article{9517f6fc-b471-4e03-be39-d3cf4c6061a1,
  abstract     = {Background and Purpose-Elevated levels of endothelin 1 (ET 1) have been reported in cerebral ischemia A role for ET may prove more important if the vascular receptors were changed We addressed whether there is any change in ET receptor expression in cerebral ischemia Methods-The right middle cerebral artery (MCA) was occluded in male Wistar rats for 2 hours with the intraluminal filament method The basilar artery and both MCAs were removed after 46 hours of recirculation The contractile responses to ET 1 a combined ETA and ETB receptor agonist and sarafotoxin 6c (S6c) a selective ETB receptor agonist were examined in vitro and ET receptor mRNA was quantified by real time polymerase chain reaction Results-S6c which had no contractile effect per se on fresh or sham operated rat cerebral arteries induced a marked contraction in the occluded MCA (E-m [maximum contraction calculated as percentage of the contractile capacity of 63 5 mmol/L K+] =68+/-68% P&lt;0 0001) while there was no difference in the responses to ET 1 after cerebral ischemia Real time polymerase chain reaction revealed a significant upregulation of both the ETA and ETB receptors (both P&lt;0 05) in the occluded MCA compared with the nonoccluded MCA from the same rats Conclusions-Focal cerebral ischemia in rat induces increased transcription of both ETA and ETB receptors which results in the appearance of a contractile response to the ETB receptor agonist S6c These results suggest a role for ET receptors in the pathogenesis of a vascular component after cerebral ischemia.},
  author       = {Stenman, Emelie and Malmsjö, Malin and Uddman, Erik and Gidö, Gunilla and Wieloch, Tadeusz and Edvinsson, Lars},
  issn         = {1524-4628},
  keyword      = {receptors,endothelins,middle cerebral artery,cerebral ischemia,endothelin,rats},
  language     = {eng},
  number       = {9},
  pages        = {2311--2316},
  publisher    = {American Heart Association},
  series       = { Stroke: a journal of cerebral circulation},
  title        = {Cerebral ischemia upregulates vascular endothelin ETB receptors in rat},
  url          = {http://dx.doi.org/10.1161/01.STR.0000028183.04277.32},
  volume       = {33},
  year         = {2002},
}