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Gene delivery to adult neural stem cells

Falk, A; Holmstrom, N; Carlen, M; Cassidy, R; Lundberg, Cecilia LU and Frisen, J (2002) In Experimental Cell Research 279(1). p.34-39
Abstract
Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to... (More)
Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to neural stem or progenitor cells by the use of specific promoters. These techniques may be utilized both to study the function of various genes in the differentiation of neural stem cells to specific cell fates and, ultimately, for gene therapy or to generate specific differentiated progeny for cell transplantation. (C) 2002 Elsevier Science (USA). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Cell Research
volume
279
issue
1
pages
34 - 39
publisher
Academic Press
external identifiers
  • wos:000177968800004
  • pmid:12213211
  • scopus:0036382924
ISSN
1090-2422
DOI
10.1006/excr.2002.5569
language
English
LU publication?
yes
id
085ad1e8-8a03-44a2-8472-fa6fea46966c (old id 329222)
date added to LUP
2007-08-17 11:48:40
date last changed
2017-04-16 03:36:16
@article{085ad1e8-8a03-44a2-8472-fa6fea46966c,
  abstract     = {Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to neural stem or progenitor cells by the use of specific promoters. These techniques may be utilized both to study the function of various genes in the differentiation of neural stem cells to specific cell fates and, ultimately, for gene therapy or to generate specific differentiated progeny for cell transplantation. (C) 2002 Elsevier Science (USA).},
  author       = {Falk, A and Holmstrom, N and Carlen, M and Cassidy, R and Lundberg, Cecilia and Frisen, J},
  issn         = {1090-2422},
  language     = {eng},
  number       = {1},
  pages        = {34--39},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Gene delivery to adult neural stem cells},
  url          = {http://dx.doi.org/10.1006/excr.2002.5569},
  volume       = {279},
  year         = {2002},
}