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Formation and conversion of oxygen metabolites by Lactococcus lactis subsp lactis ATCC 19435 under different growth conditions

van Niel, Ed LU ; Hofvendahl, Karin LU and Hahn-Hägerdal, Bärbel LU (2002) In Applied and Environmental Microbiology 68(9). p.4350-4356
Abstract
A semidefined medium based on Casamino Acids allowed Lactococcus lactis ATCC 19435 to grow in the presence of oxygen at a slow rate (0.015 h(-1)). Accumulation of H2O2 in the culture prevented a higher growth rate. Addition of asparagine to the medium increased the growth rate; whereby H2O2 accumulated only temporarily during the lag phase. H2O2 is an inhibitor for several glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase being the most sensitive. Strain ATCC 19435 contained NADH oxidase (maximum specific rate under aerobic conditions, 426 nmol of NADH min(-1) mg of protein(-1)), which reduced oxygen to water, whereby superoxide was formed as a by-product. H2O2 originated from the dismutation of superoxide by superoxide... (More)
A semidefined medium based on Casamino Acids allowed Lactococcus lactis ATCC 19435 to grow in the presence of oxygen at a slow rate (0.015 h(-1)). Accumulation of H2O2 in the culture prevented a higher growth rate. Addition of asparagine to the medium increased the growth rate; whereby H2O2 accumulated only temporarily during the lag phase. H2O2 is an inhibitor for several glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase being the most sensitive. Strain ATCC 19435 contained NADH oxidase (maximum specific rate under aerobic conditions, 426 nmol of NADH min(-1) mg of protein(-1)), which reduced oxygen to water, whereby superoxide was formed as a by-product. H2O2 originated from the dismutation of superoxide by superoxide dismutase. Although H2O2, was rapidly destroyed under high metabolic fluxes, neither NADH peroxidase nor any other enzymatic H2O2-reducing activity was detected. However, pyruvate, the end product of glycolysis; reacted nonenzymatically and rapidly with H2O2 and hence was a potential alternative for scavenging of this oxygen metabolite intracellularly. Indeed, intracellular concentrations of up to 93 mM pyruvate were detected in aerobic cultures growing at high rates. It is hypothesized that self-generated pyruvate may serve to protect L. lactis strain ATCC 19435 from H2O2. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
68
issue
9
pages
4350 - 4356
publisher
American Society for Microbiology
external identifiers
  • pmid:12200286
  • wos:000177718000025
  • scopus:0036729482
ISSN
0099-2240
DOI
10.1128/AEM.68.9.4350-4356.2002
language
English
LU publication?
yes
id
5e74e432-9dd1-49fa-91dd-5e449d216083 (old id 329902)
date added to LUP
2007-11-13 13:25:10
date last changed
2017-02-26 03:33:46
@article{5e74e432-9dd1-49fa-91dd-5e449d216083,
  abstract     = {A semidefined medium based on Casamino Acids allowed Lactococcus lactis ATCC 19435 to grow in the presence of oxygen at a slow rate (0.015 h(-1)). Accumulation of H2O2 in the culture prevented a higher growth rate. Addition of asparagine to the medium increased the growth rate; whereby H2O2 accumulated only temporarily during the lag phase. H2O2 is an inhibitor for several glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase being the most sensitive. Strain ATCC 19435 contained NADH oxidase (maximum specific rate under aerobic conditions, 426 nmol of NADH min(-1) mg of protein(-1)), which reduced oxygen to water, whereby superoxide was formed as a by-product. H2O2 originated from the dismutation of superoxide by superoxide dismutase. Although H2O2, was rapidly destroyed under high metabolic fluxes, neither NADH peroxidase nor any other enzymatic H2O2-reducing activity was detected. However, pyruvate, the end product of glycolysis; reacted nonenzymatically and rapidly with H2O2 and hence was a potential alternative for scavenging of this oxygen metabolite intracellularly. Indeed, intracellular concentrations of up to 93 mM pyruvate were detected in aerobic cultures growing at high rates. It is hypothesized that self-generated pyruvate may serve to protect L. lactis strain ATCC 19435 from H2O2.},
  author       = {van Niel, Ed and Hofvendahl, Karin and Hahn-Hägerdal, Bärbel},
  issn         = {0099-2240},
  language     = {eng},
  number       = {9},
  pages        = {4350--4356},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Formation and conversion of oxygen metabolites by Lactococcus lactis subsp lactis ATCC 19435 under different growth conditions},
  url          = {http://dx.doi.org/10.1128/AEM.68.9.4350-4356.2002},
  volume       = {68},
  year         = {2002},
}