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Influence of elevated CO2 on the fungal community in a coastal scrub oak forest soil investigated with terminal-restriction fragment length polymorphism analysis

Klamer, Morten LU ; Roberts, MS; Levine, LH; Drake, BG and Garland, JL (2002) In Applied and Environmental Microbiology 68(9). p.4370-4376
Abstract
Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions,... (More)
Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions, a significantly increased level of ergosterol was detected in the elevated CO, treatments relative to ambient controls. Fungal community composition was determined by terminal-restriction fragment length polymorphism (T-RFLP) analysis of the internal transcribed spacer (ITS) region. The specificities of different ITS primer sets were evaluated against plant and fungal species isolated from the experimental site. Changes in community composition were assessed by principal component analyses of T-RFLP profiles resolved by capillary electrophoresis. Fungal species richness, defined by the total number of terminal restriction fragments, was not significantly affected by either CO2 treatment or soil fraction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
68
issue
9
pages
4370 - 4376
publisher
American Society for Microbiology
external identifiers
  • wos:000177718000028
  • pmid:12200289
  • scopus:0036729772
ISSN
0099-2240
DOI
10.1128/AEM.68.9.4370-4376.2002
language
English
LU publication?
yes
id
b5d1adbb-3fc4-4497-82ba-6f36f91f1505 (old id 329906)
date added to LUP
2007-11-07 14:11:55
date last changed
2017-11-12 03:31:21
@article{b5d1adbb-3fc4-4497-82ba-6f36f91f1505,
  abstract     = {Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions, a significantly increased level of ergosterol was detected in the elevated CO, treatments relative to ambient controls. Fungal community composition was determined by terminal-restriction fragment length polymorphism (T-RFLP) analysis of the internal transcribed spacer (ITS) region. The specificities of different ITS primer sets were evaluated against plant and fungal species isolated from the experimental site. Changes in community composition were assessed by principal component analyses of T-RFLP profiles resolved by capillary electrophoresis. Fungal species richness, defined by the total number of terminal restriction fragments, was not significantly affected by either CO2 treatment or soil fraction.},
  author       = {Klamer, Morten and Roberts, MS and Levine, LH and Drake, BG and Garland, JL},
  issn         = {0099-2240},
  language     = {eng},
  number       = {9},
  pages        = {4370--4376},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Influence of elevated CO2 on the fungal community in a coastal scrub oak forest soil investigated with terminal-restriction fragment length polymorphism analysis},
  url          = {http://dx.doi.org/10.1128/AEM.68.9.4370-4376.2002},
  volume       = {68},
  year         = {2002},
}