Influence of elevated CO2 on the fungal community in a coastal scrub oak forest soil investigated with terminal-restriction fragment length polymorphism analysis
(2002) In Applied and Environmental Microbiology 68(9). p.4370-4376- Abstract
- Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions,... (More)
- Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions, a significantly increased level of ergosterol was detected in the elevated CO, treatments relative to ambient controls. Fungal community composition was determined by terminal-restriction fragment length polymorphism (T-RFLP) analysis of the internal transcribed spacer (ITS) region. The specificities of different ITS primer sets were evaluated against plant and fungal species isolated from the experimental site. Changes in community composition were assessed by principal component analyses of T-RFLP profiles resolved by capillary electrophoresis. Fungal species richness, defined by the total number of terminal restriction fragments, was not significantly affected by either CO2 treatment or soil fraction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/329906
- author
- Klamer, Morten LU ; Roberts, MS ; Levine, LH ; Drake, BG and Garland, JL
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 68
- issue
- 9
- pages
- 4370 - 4376
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000177718000028
- pmid:12200289
- scopus:0036729772
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.68.9.4370-4376.2002
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Ecology (Closed 2011) (011006010)
- id
- b5d1adbb-3fc4-4497-82ba-6f36f91f1505 (old id 329906)
- date added to LUP
- 2016-04-01 12:30:45
- date last changed
- 2022-03-13 18:54:48
@article{b5d1adbb-3fc4-4497-82ba-6f36f91f1505, abstract = {{Sixteen open-top chambers (diameter, 3.66 m) were established in a scrub oak habitat in central Florida where vegetation was removed in a planned burn prior to chamber installation. Eight control chambers have been continuously exposed to ambient air and eight have been continuously exposed to elevated CO2 at twice-ambient concentration (similar to700 ppm) for 5 years. Soil cores were collected from each chamber to examine the influence of elevated atmospheric CO, on the fungal community in different soil fractions. Each soil sample was physically fractionated into bulk soil, rhizosphere soil, and roots for separate analyses. Changes in relative fungal biomass were estimated by the ergosterol technique. In the bulk soil and root fractions, a significantly increased level of ergosterol was detected in the elevated CO, treatments relative to ambient controls. Fungal community composition was determined by terminal-restriction fragment length polymorphism (T-RFLP) analysis of the internal transcribed spacer (ITS) region. The specificities of different ITS primer sets were evaluated against plant and fungal species isolated from the experimental site. Changes in community composition were assessed by principal component analyses of T-RFLP profiles resolved by capillary electrophoresis. Fungal species richness, defined by the total number of terminal restriction fragments, was not significantly affected by either CO2 treatment or soil fraction.}}, author = {{Klamer, Morten and Roberts, MS and Levine, LH and Drake, BG and Garland, JL}}, issn = {{0099-2240}}, language = {{eng}}, number = {{9}}, pages = {{4370--4376}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Influence of elevated CO2 on the fungal community in a coastal scrub oak forest soil investigated with terminal-restriction fragment length polymorphism analysis}}, url = {{http://dx.doi.org/10.1128/AEM.68.9.4370-4376.2002}}, doi = {{10.1128/AEM.68.9.4370-4376.2002}}, volume = {{68}}, year = {{2002}}, }