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Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging

Hult, Jenny LU ; Dahlstrand, Ulf LU ; Merdasa, Aboma LU ; Wickerström, Karin ; Chakari, Rehan ; Persson, Bertil LU ; Cinthio, Magnus LU ; Erlöv, Tobias LU ; Albinsson, John LU and Gesslein, Bodil LU , et al. (2020) In Journal of Biophotonics 13(5).
Abstract

Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths of laser optics and ultrasound. Our aim was to determine the spectral signature of cSCC using PA imaging and to use this signature to visualize tumor architecture and borders. Two-dimensional PA images of 33 cSCCs and surrounding healthy skin were acquired ex vivo, using 59 excitation wavelengths from 680 to 970 nm. The spectral response of the cSCCs was compared to healthy tissue, and the difference was found to be... (More)

Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths of laser optics and ultrasound. Our aim was to determine the spectral signature of cSCC using PA imaging and to use this signature to visualize tumor architecture and borders. Two-dimensional PA images of 33 cSCCs and surrounding healthy skin were acquired ex vivo, using 59 excitation wavelengths from 680 to 970 nm. The spectral response of the cSCCs was compared to healthy tissue, and the difference was found to be greatest at wavelengths in the range 765 to 960 nm (P <.05). Three-dimensional PA images were constructed from spectra obtained in the y-z plane using a linear stepper motor moving along the x-plane. Spectral unmixing was then performed which provided a clear three-dimensional view of the distribution of tumor masses and their borders.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biophotonics
volume
13
issue
5
article number
e201960212
publisher
John Wiley and Sons
external identifiers
  • pmid:32049420
  • scopus:85079711594
ISSN
1864-063X
DOI
10.1002/jbio.201960212
language
English
LU publication?
yes
id
32b35ea7-3c90-44b2-a183-554e021a2d0e
date added to LUP
2020-03-05 11:47:18
date last changed
2021-02-17 01:21:18
@article{32b35ea7-3c90-44b2-a183-554e021a2d0e,
  abstract     = {<p>Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths of laser optics and ultrasound. Our aim was to determine the spectral signature of cSCC using PA imaging and to use this signature to visualize tumor architecture and borders. Two-dimensional PA images of 33 cSCCs and surrounding healthy skin were acquired ex vivo, using 59 excitation wavelengths from 680 to 970 nm. The spectral response of the cSCCs was compared to healthy tissue, and the difference was found to be greatest at wavelengths in the range 765 to 960 nm (P &lt;.05). Three-dimensional PA images were constructed from spectra obtained in the y-z plane using a linear stepper motor moving along the x-plane. Spectral unmixing was then performed which provided a clear three-dimensional view of the distribution of tumor masses and their borders.</p>},
  author       = {Hult, Jenny and Dahlstrand, Ulf and Merdasa, Aboma and Wickerström, Karin and Chakari, Rehan and Persson, Bertil and Cinthio, Magnus and Erlöv, Tobias and Albinsson, John and Gesslein, Bodil and Sheikh, Rafi and Malmsjö, Malin},
  issn         = {1864-063X},
  language     = {eng},
  number       = {5},
  publisher    = {John Wiley and Sons},
  series       = {Journal of Biophotonics},
  title        = {Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging},
  url          = {http://dx.doi.org/10.1002/jbio.201960212},
  doi          = {10.1002/jbio.201960212},
  volume       = {13},
  year         = {2020},
}