Advanced

Relationship between production of the phytotoxin prehelminthosporol and virulence in isolates of the plant pathogenic fungus Bipolaris sorokiniana

Apoga, D; Akesson, H; Jansson, Hans-Börje LU and Odham, G (2002) In European Journal of Plant Pathology 108(6). p.519-526
Abstract
A gas chromatographic method was developed to quantify the phytotoxin prehelminthosporol, which is a sesquiterpene metabolite of the plant pathogen Bipolaris sorokiniana. The toxin was extracted from mycelium or culture filtrates, pre-cleaned using solid phase extraction, and analyzed by gas chromatography as a trimethylsilyl-derivative. The detection limit of the method was 5 ng mul(-)1 (signal to noise ratio 4 : 1) which corresponds to ca. 15 ng prehelminthosporol per mg dry weight of mycelium or 15 ng prehelminthosporol per ml culture filtrate. The total amount of prehelminthosporol (mycelium plus culture filtrate) increased with cultivation time when examined in six isolates of B. sorokiniana after 6, 9, 12 and 15 days of incubation.... (More)
A gas chromatographic method was developed to quantify the phytotoxin prehelminthosporol, which is a sesquiterpene metabolite of the plant pathogen Bipolaris sorokiniana. The toxin was extracted from mycelium or culture filtrates, pre-cleaned using solid phase extraction, and analyzed by gas chromatography as a trimethylsilyl-derivative. The detection limit of the method was 5 ng mul(-)1 (signal to noise ratio 4 : 1) which corresponds to ca. 15 ng prehelminthosporol per mg dry weight of mycelium or 15 ng prehelminthosporol per ml culture filtrate. The total amount of prehelminthosporol (mycelium plus culture filtrate) increased with cultivation time when examined in six isolates of B. sorokiniana after 6, 9, 12 and 15 days of incubation. The screening experiment of 17 isolates for prehelminthosporol production after 8 days of incubation revealed significant differences in the toxin production between the isolates. The isolates with low toxin production had lower virulence towards barley roots compared to those with higher production of the toxin. However, the virulence did not increase with prehelminthosporol level among the high producing isolates. Prehelminthosporol was also analyzed in a number of related Bipolaris and Drechslera species. In addition to B. sorokiniana, three out of six Bipolaris species (B. setariae, B. zeicola, B. victoriae) produced prehelminthosporol, which indicates that ability to produce prehelminthosporol is conserved among closely-related Bipolaris species. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Helminthosporium sativum, Cochliobolus sativus, Bipolaris zeicola, Bipolaris setariae, Bipolaris victoriae, virulence, toxin, Hordeum vulgare
in
European Journal of Plant Pathology
volume
108
issue
6
pages
519 - 526
publisher
Kluwer
external identifiers
  • wos:000177720900004
  • scopus:0036658275
ISSN
0929-1873
language
English
LU publication?
yes
id
f3547ddd-912e-4444-8696-98e6f58430e4 (old id 330110)
date added to LUP
2007-08-07 15:25:22
date last changed
2017-05-21 03:47:27
@article{f3547ddd-912e-4444-8696-98e6f58430e4,
  abstract     = {A gas chromatographic method was developed to quantify the phytotoxin prehelminthosporol, which is a sesquiterpene metabolite of the plant pathogen Bipolaris sorokiniana. The toxin was extracted from mycelium or culture filtrates, pre-cleaned using solid phase extraction, and analyzed by gas chromatography as a trimethylsilyl-derivative. The detection limit of the method was 5 ng mul(-)1 (signal to noise ratio 4 : 1) which corresponds to ca. 15 ng prehelminthosporol per mg dry weight of mycelium or 15 ng prehelminthosporol per ml culture filtrate. The total amount of prehelminthosporol (mycelium plus culture filtrate) increased with cultivation time when examined in six isolates of B. sorokiniana after 6, 9, 12 and 15 days of incubation. The screening experiment of 17 isolates for prehelminthosporol production after 8 days of incubation revealed significant differences in the toxin production between the isolates. The isolates with low toxin production had lower virulence towards barley roots compared to those with higher production of the toxin. However, the virulence did not increase with prehelminthosporol level among the high producing isolates. Prehelminthosporol was also analyzed in a number of related Bipolaris and Drechslera species. In addition to B. sorokiniana, three out of six Bipolaris species (B. setariae, B. zeicola, B. victoriae) produced prehelminthosporol, which indicates that ability to produce prehelminthosporol is conserved among closely-related Bipolaris species.},
  author       = {Apoga, D and Akesson, H and Jansson, Hans-Börje and Odham, G},
  issn         = {0929-1873},
  keyword      = {Helminthosporium sativum,Cochliobolus sativus,Bipolaris zeicola,Bipolaris setariae,Bipolaris victoriae,virulence,toxin,Hordeum vulgare},
  language     = {eng},
  number       = {6},
  pages        = {519--526},
  publisher    = {Kluwer},
  series       = {European Journal of Plant Pathology},
  title        = {Relationship between production of the phytotoxin prehelminthosporol and virulence in isolates of the plant pathogenic fungus Bipolaris sorokiniana},
  volume       = {108},
  year         = {2002},
}