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The temperature influences the ratio of glucosidase and galactosidase activities of beta-glycosidases

Hansson, Therese LU and Adlercreutz, Patrick LU (2002) In Biotechnology Letters 24(18). p.1465-1471
Abstract
The selectivity for the glycon part of a donor substrate of beta-glycosidases from almond, a mesophilic (Kluyveromyces fragilis) and three highly thermophilic organisms (Caldocellum saccharolyticum, Sulfolobus solfataricus and Pyrococcus furiosus) was investigated at various temperatures (25-90 degreesC ). On the basis of kinetic constants, the selectivity was calculated as the specificity constant (V-max/K-m) ratio or V-max ratio of glucoside to galactoside donor. In the almond beta-glucosidase and the mesostable enzyme one enzyme activity dominated whereas the thermostable enzymes expressed both high beta-glucosidase and high beta-galactosidase activities. Surprisingly, for beta-glycosidases from almond, K. fragilis, and C.... (More)
The selectivity for the glycon part of a donor substrate of beta-glycosidases from almond, a mesophilic (Kluyveromyces fragilis) and three highly thermophilic organisms (Caldocellum saccharolyticum, Sulfolobus solfataricus and Pyrococcus furiosus) was investigated at various temperatures (25-90 degreesC ). On the basis of kinetic constants, the selectivity was calculated as the specificity constant (V-max/K-m) ratio or V-max ratio of glucoside to galactoside donor. In the almond beta-glucosidase and the mesostable enzyme one enzyme activity dominated whereas the thermostable enzymes expressed both high beta-glucosidase and high beta-galactosidase activities. Surprisingly, for beta-glycosidases from almond, K. fragilis, and C. saccharolyticum the donor selectivity decreased as the temperature increased. In contrast, two of the highly thermostable enzymes (from S. solfataricus and P. furiosus) had constant donor selectivity as the temperature increased. The results thus showed beta-glycosidases of differing origins to differ markedly in their substrate specificity and in the extent to which their selectivity for the glycon part of the donor substrate is influenced by the temperature. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
substrate specificity, temperature, kinetics, beta-glycosidase, donor selectivity
in
Biotechnology Letters
volume
24
issue
18
pages
1465 - 1471
publisher
Springer
external identifiers
  • wos:000177576600002
  • scopus:0036732504
ISSN
1573-6776
DOI
10.1023/A:1019875116426
language
English
LU publication?
yes
id
707dafa3-45d1-4e79-8b47-35a35b190c1c (old id 330174)
date added to LUP
2007-08-24 13:51:07
date last changed
2017-01-01 06:50:35
@article{707dafa3-45d1-4e79-8b47-35a35b190c1c,
  abstract     = {The selectivity for the glycon part of a donor substrate of beta-glycosidases from almond, a mesophilic (Kluyveromyces fragilis) and three highly thermophilic organisms (Caldocellum saccharolyticum, Sulfolobus solfataricus and Pyrococcus furiosus) was investigated at various temperatures (25-90 degreesC ). On the basis of kinetic constants, the selectivity was calculated as the specificity constant (V-max/K-m) ratio or V-max ratio of glucoside to galactoside donor. In the almond beta-glucosidase and the mesostable enzyme one enzyme activity dominated whereas the thermostable enzymes expressed both high beta-glucosidase and high beta-galactosidase activities. Surprisingly, for beta-glycosidases from almond, K. fragilis, and C. saccharolyticum the donor selectivity decreased as the temperature increased. In contrast, two of the highly thermostable enzymes (from S. solfataricus and P. furiosus) had constant donor selectivity as the temperature increased. The results thus showed beta-glycosidases of differing origins to differ markedly in their substrate specificity and in the extent to which their selectivity for the glycon part of the donor substrate is influenced by the temperature.},
  author       = {Hansson, Therese and Adlercreutz, Patrick},
  issn         = {1573-6776},
  keyword      = {substrate specificity,temperature,kinetics,beta-glycosidase,donor selectivity},
  language     = {eng},
  number       = {18},
  pages        = {1465--1471},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {The temperature influences the ratio of glucosidase and galactosidase activities of beta-glycosidases},
  url          = {http://dx.doi.org/10.1023/A:1019875116426},
  volume       = {24},
  year         = {2002},
}