RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells
(2002) In Journal of Orthopaedic Research 20(4). p.723-729- Abstract
- Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via its N-terminal domain and to the transcription and translation factor Y-box binding protein-1 (YB-1) through its C-terminal domain. The liposarcoma fusion protein TLS/CHOP retains the ability to bind RNA Pol II but lacks the ability to recruit YB-1 due to replacement of the C-terminal domain of TLS by CHOP. In an in vivo splicing... (More)
- Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via its N-terminal domain and to the transcription and translation factor Y-box binding protein-1 (YB-1) through its C-terminal domain. The liposarcoma fusion protein TLS/CHOP retains the ability to bind RNA Pol II but lacks the ability to recruit YB-1 due to replacement of the C-terminal domain of TLS by CHOP. In an in vivo splicing assay, YB-1 promotes splicing of adenovirus E1A pre-mRNA predominantly to the 13S isoform. The oncogenic TLS/CHOP fusion protein inhibits this splicing function of YB-1 in a dominant negative manner. When considered in conjunction with studies on other sarcoma fusion proteins, these data suggest that aberrant RNA splicing may be a common feature of human sarcomas. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/331972
- author
- Rapp, TB ; Yang, L ; Conrad, EU ; Mandahl, Nils LU and Chansky, HA
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- RNA splicing, translocation, fusion protein, TLS/CHOP, sarcoma, YB-1
- in
- Journal of Orthopaedic Research
- volume
- 20
- issue
- 4
- pages
- 723 - 729
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000177191600012
- pmid:12168660
- scopus:0036319256
- ISSN
- 1554-527X
- DOI
- 10.1016/S0736-0266(02)00006-2
- language
- English
- LU publication?
- yes
- id
- f738e7ea-2957-4249-9a0e-3160e9eaf47e (old id 331972)
- date added to LUP
- 2016-04-01 11:55:31
- date last changed
- 2022-02-03 07:03:09
@article{f738e7ea-2957-4249-9a0e-3160e9eaf47e,
abstract = {{Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via its N-terminal domain and to the transcription and translation factor Y-box binding protein-1 (YB-1) through its C-terminal domain. The liposarcoma fusion protein TLS/CHOP retains the ability to bind RNA Pol II but lacks the ability to recruit YB-1 due to replacement of the C-terminal domain of TLS by CHOP. In an in vivo splicing assay, YB-1 promotes splicing of adenovirus E1A pre-mRNA predominantly to the 13S isoform. The oncogenic TLS/CHOP fusion protein inhibits this splicing function of YB-1 in a dominant negative manner. When considered in conjunction with studies on other sarcoma fusion proteins, these data suggest that aberrant RNA splicing may be a common feature of human sarcomas. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.}},
author = {{Rapp, TB and Yang, L and Conrad, EU and Mandahl, Nils and Chansky, HA}},
issn = {{1554-527X}},
keywords = {{RNA splicing; translocation; fusion protein; TLS/CHOP; sarcoma; YB-1}},
language = {{eng}},
number = {{4}},
pages = {{723--729}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Orthopaedic Research}},
title = {{RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells}},
url = {{http://dx.doi.org/10.1016/S0736-0266(02)00006-2}},
doi = {{10.1016/S0736-0266(02)00006-2}},
volume = {{20}},
year = {{2002}},
}