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Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy

Haberkorn, M; Frank, J; Harasek, M; Nilsson, Johan LU ; Laurell, Thomas LU and Lendl, B (2002) In Applied Spectroscopy 56(7). p.902-908
Abstract
A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the... (More)
A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the picoliter range. Moreover, an increase in sensitivity for FT-IR measurements could be achieved, lowering the mass detection limit of sugars (such as the investigated sucrose) to 53 picograms. As is demonstrated on the example of an HPLC separation of a mixture of glucose and fructose, interfacing LC systems to FT-IR using a piezoactuated flow-through microdispenser is a feasible and promising approach. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
micro-liquid handling, FT-IR, infrared spectroscopy, Fourier transform, flow-through microdispenser, solvent elimination
in
Applied Spectroscopy
volume
56
issue
7
pages
902 - 908
publisher
Society for Applied Spectroscopy
external identifiers
  • wos:000177139500015
  • scopus:0036649651
ISSN
1943-3530
DOI
10.1366/000370202760171581
language
English
LU publication?
yes
id
be39a9f0-70e1-4339-aa16-7cd53d48c28c (old id 332457)
date added to LUP
2007-08-22 15:20:38
date last changed
2017-01-01 05:09:08
@article{be39a9f0-70e1-4339-aa16-7cd53d48c28c,
  abstract     = {A new interface for FT-IR analysis of liquid samples on the basis of solvent elimination is presented. The approach is based on a piezoactuated flow-through microdispenser, a device built of two microstructured silicon wafers designed for micro-liquid handling. It could be verified during preliminary studies using a sequential injection (SI) system for automated liquid handling that the flow-through microdispenser as a possible interface for flow system-FT-IR analysis has the capability of meeting the demands of hyphenated miniaturized liquid handling systems (e.g., mu-HPLC, microhigh performance liquid chromatography), as it successfully provides highly stable, reliable and reproducible operating conditions for liquid handling in the picoliter range. Moreover, an increase in sensitivity for FT-IR measurements could be achieved, lowering the mass detection limit of sugars (such as the investigated sucrose) to 53 picograms. As is demonstrated on the example of an HPLC separation of a mixture of glucose and fructose, interfacing LC systems to FT-IR using a piezoactuated flow-through microdispenser is a feasible and promising approach.},
  author       = {Haberkorn, M and Frank, J and Harasek, M and Nilsson, Johan and Laurell, Thomas and Lendl, B},
  issn         = {1943-3530},
  keyword      = {micro-liquid handling,FT-IR,infrared spectroscopy,Fourier transform,flow-through microdispenser,solvent elimination},
  language     = {eng},
  number       = {7},
  pages        = {902--908},
  publisher    = {Society for Applied Spectroscopy},
  series       = {Applied Spectroscopy},
  title        = {Flow-through picoliter dispenser: A new approach for solvent elimination in FT-IR spectroscopy},
  url          = {http://dx.doi.org/10.1366/000370202760171581},
  volume       = {56},
  year         = {2002},
}