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Transendothelial transport of low-density lipoprotein and albumin across the rat peritoneum in vivo: Effects of the transcytosis inhibitors NEM and filipin

Rosengren, Bert-Inge LU ; Al Rayyes, O and Rippe, Bengt LU (2002) In Journal of Vascular Research1992-01-01+01:00 39(3). p.230-237
Abstract
This study was performed to investigate the mechanisms responsible for the transport of albumin and low-density lipoprotein (LDL) across capillary walls in vivo. To separate transcytosis from passive, 'porous' transport, we tested the effects of the transcytosis inhibitors N-ethylmaleimide (NEM) and filipin given intraperitoneally on the peritoneal capillary clearance of LDL and albumin in anesthetized rats undergoing peritoneal dialysis. Radiolabeled human albumin or LDL was given intraarterially, and Cr-51-EDTA was infused intravenously. A 2-hour peritoneal dialysis dwell was performed using 16 ml of conventional 1.36% glucose-based dialysis fluid. The clearance of LDL and albumin to the dialysate and the peritoneal mass transfer... (More)
This study was performed to investigate the mechanisms responsible for the transport of albumin and low-density lipoprotein (LDL) across capillary walls in vivo. To separate transcytosis from passive, 'porous' transport, we tested the effects of the transcytosis inhibitors N-ethylmaleimide (NEM) and filipin given intraperitoneally on the peritoneal capillary clearance of LDL and albumin in anesthetized rats undergoing peritoneal dialysis. Radiolabeled human albumin or LDL was given intraarterially, and Cr-51-EDTA was infused intravenously. A 2-hour peritoneal dialysis dwell was performed using 16 ml of conventional 1.36% glucose-based dialysis fluid. The clearance of LDL and albumin to the dialysate and the peritoneal mass transfer coefficient for Cr-51-EDTA were assessed. Following intraperitoneal NEM incubations (0.5-5 mM), there were marked increases in the peritoneal transport of albumin and LDL for NEM doses exceeding 1 mM. For lower NEM doses, there were no reductions in clearance. Filipin incubations (0.24 ug/ml) did not affect the clearance of either macromolecule. In conclusion, neither NEM nor filipin caused reductions in albumin or LDL clearance across the peritoneal capillaries. The present data clearly show that NEM and filipin are unsuitable as transcytosis inhibitors in vivo. Copyright (C) 2002 S. Karger AG, Basel. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
peritoneal dialysis, endothelium, active transport, capillary permeability, passive transport, pores, vesicles
in
Journal of Vascular Research1992-01-01+01:00
volume
39
issue
3
pages
230 - 237
publisher
Karger
external identifiers
  • wos:000176950900004
  • scopus:0036318993
ISSN
1423-0135
DOI
10.1159/000063688
language
English
LU publication?
yes
id
4da7576a-7ed1-4aa8-9dce-6de4996005f4 (old id 332656)
date added to LUP
2007-11-12 12:44:40
date last changed
2017-01-01 06:48:31
@article{4da7576a-7ed1-4aa8-9dce-6de4996005f4,
  abstract     = {This study was performed to investigate the mechanisms responsible for the transport of albumin and low-density lipoprotein (LDL) across capillary walls in vivo. To separate transcytosis from passive, 'porous' transport, we tested the effects of the transcytosis inhibitors N-ethylmaleimide (NEM) and filipin given intraperitoneally on the peritoneal capillary clearance of LDL and albumin in anesthetized rats undergoing peritoneal dialysis. Radiolabeled human albumin or LDL was given intraarterially, and Cr-51-EDTA was infused intravenously. A 2-hour peritoneal dialysis dwell was performed using 16 ml of conventional 1.36% glucose-based dialysis fluid. The clearance of LDL and albumin to the dialysate and the peritoneal mass transfer coefficient for Cr-51-EDTA were assessed. Following intraperitoneal NEM incubations (0.5-5 mM), there were marked increases in the peritoneal transport of albumin and LDL for NEM doses exceeding 1 mM. For lower NEM doses, there were no reductions in clearance. Filipin incubations (0.24 ug/ml) did not affect the clearance of either macromolecule. In conclusion, neither NEM nor filipin caused reductions in albumin or LDL clearance across the peritoneal capillaries. The present data clearly show that NEM and filipin are unsuitable as transcytosis inhibitors in vivo. Copyright (C) 2002 S. Karger AG, Basel.},
  author       = {Rosengren, Bert-Inge and Al Rayyes, O and Rippe, Bengt},
  issn         = {1423-0135},
  keyword      = {peritoneal dialysis,endothelium,active transport,capillary permeability,passive transport,pores,vesicles},
  language     = {eng},
  number       = {3},
  pages        = {230--237},
  publisher    = {Karger},
  series       = {Journal of Vascular Research1992-01-01+01:00},
  title        = {Transendothelial transport of low-density lipoprotein and albumin across the rat peritoneum in vivo: Effects of the transcytosis inhibitors NEM and filipin},
  url          = {http://dx.doi.org/10.1159/000063688},
  volume       = {39},
  year         = {2002},
}