Advanced

Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation

Niculescu, M; Erichsen, T; Sukharev, V; Kerenyi, Z; Csöregi, Elisabeth LU and Schuhmann, W (2002) In Analytica Chimica Acta 463(1). p.39-51
Abstract
This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection... (More)
This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
process control, on-line, fermentation, biosensor, alcohol dehydrogenase, ethanol, redox polymer, sequential-injection analysis
in
Analytica Chimica Acta
volume
463
issue
1
pages
39 - 51
publisher
Elsevier
external identifiers
  • wos:000176875600006
  • scopus:0037118792
ISSN
1873-4324
DOI
10.1016/S0003-2670(02)00344-6
language
English
LU publication?
yes
id
a8398e19-ad39-4529-8431-d414b99121a7 (old id 333451)
date added to LUP
2007-11-16 16:26:37
date last changed
2017-02-19 04:18:32
@article{a8398e19-ad39-4529-8431-d414b99121a7,
  abstract     = {This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes.},
  author       = {Niculescu, M and Erichsen, T and Sukharev, V and Kerenyi, Z and Csöregi, Elisabeth and Schuhmann, W},
  issn         = {1873-4324},
  keyword      = {process control,on-line,fermentation,biosensor,alcohol dehydrogenase,ethanol,redox polymer,sequential-injection analysis},
  language     = {eng},
  number       = {1},
  pages        = {39--51},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation},
  url          = {http://dx.doi.org/10.1016/S0003-2670(02)00344-6},
  volume       = {463},
  year         = {2002},
}