Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation
(2002) In Analytica Chimica Acta 463(1). p.39-51- Abstract
- This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection... (More)
- This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/333451
- author
- Niculescu, M ; Erichsen, T ; Sukharev, V ; Kerenyi, Z ; Csöregi, Elisabeth LU and Schuhmann, W
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- process control, on-line, fermentation, biosensor, alcohol dehydrogenase, ethanol, redox polymer, sequential-injection analysis
- in
- Analytica Chimica Acta
- volume
- 463
- issue
- 1
- pages
- 39 - 51
- publisher
- Elsevier
- external identifiers
-
- wos:000176875600006
- scopus:0037118792
- ISSN
- 1873-4324
- DOI
- 10.1016/S0003-2670(02)00344-6
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- a8398e19-ad39-4529-8431-d414b99121a7 (old id 333451)
- date added to LUP
- 2016-04-01 17:06:23
- date last changed
- 2022-04-07 20:53:36
@article{a8398e19-ad39-4529-8431-d414b99121a7,
abstract = {{This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes.}},
author = {{Niculescu, M and Erichsen, T and Sukharev, V and Kerenyi, Z and Csöregi, Elisabeth and Schuhmann, W}},
issn = {{1873-4324}},
keywords = {{process control; on-line; fermentation; biosensor; alcohol dehydrogenase; ethanol; redox polymer; sequential-injection analysis}},
language = {{eng}},
number = {{1}},
pages = {{39--51}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation}},
url = {{http://dx.doi.org/10.1016/S0003-2670(02)00344-6}},
doi = {{10.1016/S0003-2670(02)00344-6}},
volume = {{463}},
year = {{2002}},
}