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Modulation of postendocytic sorting of G-protein coupled receptors

Whistler, JL; Enquist, Johan LU ; Marley, A; Fong, J; Gladher, F; Tsuruda, P; Murray, SR and von Zastro, M (2002) In Science 297(5581). p.615-620
Abstract
Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled... (More)
Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled receptors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Science
volume
297
issue
5581
pages
615 - 620
publisher
The American Association for the Advancement of Science
external identifiers
  • wos:000177054200055
  • pmid:12142540
  • scopus:0037178754
ISSN
1095-9203
language
English
LU publication?
yes
id
03b383aa-66d6-48ba-9283-2ef4df309397 (old id 333514)
alternative location
http://stke.sciencemag.org/cgi/reprint/sci;297/5581/615.pdf
date added to LUP
2007-11-09 15:48:17
date last changed
2017-11-19 04:15:13
@article{03b383aa-66d6-48ba-9283-2ef4df309397,
  abstract     = {Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled receptors.},
  author       = {Whistler, JL and Enquist, Johan and Marley, A and Fong, J and Gladher, F and Tsuruda, P and Murray, SR and von Zastro, M},
  issn         = {1095-9203},
  language     = {eng},
  number       = {5581},
  pages        = {615--620},
  publisher    = {The American Association for the Advancement of Science},
  series       = {Science},
  title        = {Modulation of postendocytic sorting of G-protein coupled receptors},
  volume       = {297},
  year         = {2002},
}