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Effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis and differentiation in the adult rat

Komitova, M; Perfilieva, E; Mattsson, Bengt LU ; Eriksson, PS and Johansson, Barbro LU (2002) In Journal of Cerebral Blood Flow and Metabolism 22(7). p.852-860
Abstract
The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus... (More)
The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus were determined with immunohistochemistry and were quantified stereologically. Compared with sham-operated rats, rats with cortical infarcts had a five- to sixfold ipsilateral increase in BrdU-labeled cells. About 80% of the new cells were neurons. Differential postischemic housing did not influence significantly the total number Of Surviving BrdU-labeled cells or newborn neurons. However, postischemic environmental enrichment increased the ipsilateral generation of astrocytes normalizing the astrocyte-to-neuron ratio. which was significantly reduced in rats housed in standard environment postischemically. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neurogenesis, ischemia, hippocampus, adult, astroglia, rat
in
Journal of Cerebral Blood Flow and Metabolism
volume
22
issue
7
pages
852 - 860
publisher
Nature Publishing Group
external identifiers
  • wos:000176689700010
  • pmid:12142570
  • scopus:0036303480
ISSN
1559-7016
DOI
10.1097/00004647-200207000-00010
language
English
LU publication?
yes
id
288448fa-f581-4598-8847-aa90122b7e90 (old id 334142)
date added to LUP
2007-11-07 14:25:38
date last changed
2017-07-30 04:42:05
@article{288448fa-f581-4598-8847-aa90122b7e90,
  abstract     = {The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus were determined with immunohistochemistry and were quantified stereologically. Compared with sham-operated rats, rats with cortical infarcts had a five- to sixfold ipsilateral increase in BrdU-labeled cells. About 80% of the new cells were neurons. Differential postischemic housing did not influence significantly the total number Of Surviving BrdU-labeled cells or newborn neurons. However, postischemic environmental enrichment increased the ipsilateral generation of astrocytes normalizing the astrocyte-to-neuron ratio. which was significantly reduced in rats housed in standard environment postischemically.},
  author       = {Komitova, M and Perfilieva, E and Mattsson, Bengt and Eriksson, PS and Johansson, Barbro},
  issn         = {1559-7016},
  keyword      = {neurogenesis,ischemia,hippocampus,adult,astroglia,rat},
  language     = {eng},
  number       = {7},
  pages        = {852--860},
  publisher    = {Nature Publishing Group},
  series       = {Journal of Cerebral Blood Flow and Metabolism},
  title        = {Effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis and differentiation in the adult rat},
  url          = {http://dx.doi.org/10.1097/00004647-200207000-00010},
  volume       = {22},
  year         = {2002},
}