Effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis and differentiation in the adult rat
(2002) In Journal of Cerebral Blood Flow and Metabolism 22(7). p.852-860- Abstract
- The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus... (More)
- The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus were determined with immunohistochemistry and were quantified stereologically. Compared with sham-operated rats, rats with cortical infarcts had a five- to sixfold ipsilateral increase in BrdU-labeled cells. About 80% of the new cells were neurons. Differential postischemic housing did not influence significantly the total number Of Surviving BrdU-labeled cells or newborn neurons. However, postischemic environmental enrichment increased the ipsilateral generation of astrocytes normalizing the astrocyte-to-neuron ratio. which was significantly reduced in rats housed in standard environment postischemically. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/334142
- author
- Komitova, M ; Perfilieva, E ; Mattsson, Bengt LU ; Eriksson, PS and Johansson, Barbro LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- neurogenesis, ischemia, hippocampus, adult, astroglia, rat
- in
- Journal of Cerebral Blood Flow and Metabolism
- volume
- 22
- issue
- 7
- pages
- 852 - 860
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000176689700010
- pmid:12142570
- scopus:0036303480
- ISSN
- 1559-7016
- DOI
- 10.1097/00004647-200207000-00010
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Brain Research (0131000120), Neurology, Lund (013027000), Neurobiology (013212024)
- id
- 288448fa-f581-4598-8847-aa90122b7e90 (old id 334142)
- date added to LUP
- 2016-04-01 16:51:16
- date last changed
- 2022-03-07 08:47:19
@article{288448fa-f581-4598-8847-aa90122b7e90,
abstract = {{The study aimed to elucidate the effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis. A cortical infarct was induced by it permanent ligation of the middle cerebral artery distal to the striatal branches in 6-month-old spontaneously hypertensive rats. Bromodeoxyuridine (BrdU) was administered as 7 consecutive daily injections starting 24 hours after surgery and animals were housed in standard or enriched environment. Four weeks after completed BrdU administration, BrdU incorporation and its co-localization with the neuronal markers NeuN and calbindin D28k, and the astrocytic marker glial fibrillary acidic protein in the granular cell layer and sub,granular zone of the hippocampal dentate gyrus were determined with immunohistochemistry and were quantified stereologically. Compared with sham-operated rats, rats with cortical infarcts had a five- to sixfold ipsilateral increase in BrdU-labeled cells. About 80% of the new cells were neurons. Differential postischemic housing did not influence significantly the total number Of Surviving BrdU-labeled cells or newborn neurons. However, postischemic environmental enrichment increased the ipsilateral generation of astrocytes normalizing the astrocyte-to-neuron ratio. which was significantly reduced in rats housed in standard environment postischemically.}},
author = {{Komitova, M and Perfilieva, E and Mattsson, Bengt and Eriksson, PS and Johansson, Barbro}},
issn = {{1559-7016}},
keywords = {{neurogenesis; ischemia; hippocampus; adult; astroglia; rat}},
language = {{eng}},
number = {{7}},
pages = {{852--860}},
publisher = {{Nature Publishing Group}},
series = {{Journal of Cerebral Blood Flow and Metabolism}},
title = {{Effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis and differentiation in the adult rat}},
url = {{http://dx.doi.org/10.1097/00004647-200207000-00010}},
doi = {{10.1097/00004647-200207000-00010}},
volume = {{22}},
year = {{2002}},
}