Advanced

Chronic colitis is associated with a reduction of mucosal alkaline sphingomyelinase activity

Sjoqvist, U; Hertervig, Erik LU ; Nilsson, Åke LU ; Duan, Rui-Dong LU ; Ost, A; Tribukait, B and Lofberg, R (2002) In Inflammatory Bowel Diseases 8(4). p.258-263
Abstract
Background and Aims: The hydrolysis of sphingomyelin (SM) generates key molecules regulating cell growth. Animal cancer studies support an inhibitory role for this pathway in the malignant transformation of the colonic mucosa. The activity of a specific intestinal alkaline sphingomyelinase (SMase), which hydrolyzes SM, is reduced in colorectal tumors. In this study we measured alkaline SMase activity in patients with longstanding colitis and assessed if a reduction can be used as a marker in surveillance of high risk patients. Methods: Alkaline SMase activity was measured in 139 colonic biopsies from 34 patients with longstanding, extensive colitis and from I I controls. Fifteen patients had earlier diagnosis of dysplasia or DNA... (More)
Background and Aims: The hydrolysis of sphingomyelin (SM) generates key molecules regulating cell growth. Animal cancer studies support an inhibitory role for this pathway in the malignant transformation of the colonic mucosa. The activity of a specific intestinal alkaline sphingomyelinase (SMase), which hydrolyzes SM, is reduced in colorectal tumors. In this study we measured alkaline SMase activity in patients with longstanding colitis and assessed if a reduction can be used as a marker in surveillance of high risk patients. Methods: Alkaline SMase activity was measured in 139 colonic biopsies from 34 patients with longstanding, extensive colitis and from I I controls. Fifteen patients had earlier diagnosis of dysplasia or DNA aneuploidy. Alkaline SMase activity was related to histologic dysplasia and DNA aneuploidy assessed by flow cytometry, patient age, and duration of disease. Results: Alkaline SMase activity was significantly lower in the patient group with and without dysplasia compared with controls (p = 0.006). In biopsies, an association was not found between alkaline SMase activity, dysplasia, or DNA ploidy. However, alkaline SMase activity decreased with age both in patients and controls (p = 0.008). Conclusions: Reduction of alkaline SMase activity seen in colorectal cancer and adenomas is also present in patients with chronic colitis. It is not complementary to dysplasia or DNA-aneuploidy in the identification of high risk patients. The age-associated decrease of alkaline SMase activity seems to be a general phenomenon indicating premature senescence of the mucosa in longstanding colitis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
inflammatory bowel disease, DNA flow cytometry, alkaline sphingomyelinase, Crohn's disease, ulcerative colitis
in
Inflammatory Bowel Diseases
volume
8
issue
4
pages
258 - 263
publisher
John Wiley & Sons
external identifiers
  • wos:000176680000004
  • scopus:0036066564
ISSN
1536-4844
DOI
10.1097/00054725-200207000-00004
language
English
LU publication?
yes
id
22fc9327-3d61-4f84-8919-5959560f270a (old id 334162)
date added to LUP
2007-11-07 18:14:01
date last changed
2017-12-17 03:27:47
@article{22fc9327-3d61-4f84-8919-5959560f270a,
  abstract     = {Background and Aims: The hydrolysis of sphingomyelin (SM) generates key molecules regulating cell growth. Animal cancer studies support an inhibitory role for this pathway in the malignant transformation of the colonic mucosa. The activity of a specific intestinal alkaline sphingomyelinase (SMase), which hydrolyzes SM, is reduced in colorectal tumors. In this study we measured alkaline SMase activity in patients with longstanding colitis and assessed if a reduction can be used as a marker in surveillance of high risk patients. Methods: Alkaline SMase activity was measured in 139 colonic biopsies from 34 patients with longstanding, extensive colitis and from I I controls. Fifteen patients had earlier diagnosis of dysplasia or DNA aneuploidy. Alkaline SMase activity was related to histologic dysplasia and DNA aneuploidy assessed by flow cytometry, patient age, and duration of disease. Results: Alkaline SMase activity was significantly lower in the patient group with and without dysplasia compared with controls (p = 0.006). In biopsies, an association was not found between alkaline SMase activity, dysplasia, or DNA ploidy. However, alkaline SMase activity decreased with age both in patients and controls (p = 0.008). Conclusions: Reduction of alkaline SMase activity seen in colorectal cancer and adenomas is also present in patients with chronic colitis. It is not complementary to dysplasia or DNA-aneuploidy in the identification of high risk patients. The age-associated decrease of alkaline SMase activity seems to be a general phenomenon indicating premature senescence of the mucosa in longstanding colitis.},
  author       = {Sjoqvist, U and Hertervig, Erik and Nilsson, Åke and Duan, Rui-Dong and Ost, A and Tribukait, B and Lofberg, R},
  issn         = {1536-4844},
  keyword      = {inflammatory bowel disease,DNA flow cytometry,alkaline sphingomyelinase,Crohn's disease,ulcerative colitis},
  language     = {eng},
  number       = {4},
  pages        = {258--263},
  publisher    = {John Wiley & Sons},
  series       = {Inflammatory Bowel Diseases},
  title        = {Chronic colitis is associated with a reduction of mucosal alkaline sphingomyelinase activity},
  url          = {http://dx.doi.org/10.1097/00054725-200207000-00004},
  volume       = {8},
  year         = {2002},
}