Enzymatic Conversion of Different Qualities of Refined Softwood Hemicellulose Recovered from Spent Sulfite Liquor
(2022) In Molecules (Basel, Switzerland) 27(10).- Abstract
Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its... (More)
Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its enzymatic hydrolysis was assessed by reducing sugar and high-performance anion-exchange chromatography analysis. The hydrolysis of the UFR preparation with Viscozyme L or Trichoderma reesei β-mannanase TrMan5A (1 mg/mL) resulted in less than ~50% bioconversion of AcGGM. The AcGGM in the AD preparation was hydrolyzed to a higher degree (~67% with TrMan5A and 80% with Viscozyme L) and showed the highest conversion rate. This indicates that SSL contains enzyme-inhibitory compounds (e.g., lignin and lignin-derived compounds such as lignosulfonates) which were successfully removed.
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- author
- Bhattacharya, Abhishek LU ; Butler, Samuel LU ; Al-Rudainy, Basel LU ; Wallberg, Ola LU and Stålbrand, Henrik LU
- organization
- publishing date
- 2022-05-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hydrolysis, Lignin/chemistry, Polysaccharides, Sulfites
- in
- Molecules (Basel, Switzerland)
- volume
- 27
- issue
- 10
- article number
- 3207
- pages
- 20 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85131659678
- pmid:35630684
- ISSN
- 1420-3049
- DOI
- 10.3390/molecules27103207
- language
- English
- LU publication?
- yes
- id
- 334fd27a-9699-4585-9a35-1b57dbaefbbd
- date added to LUP
- 2022-06-18 11:09:28
- date last changed
- 2024-09-20 02:53:10
@article{334fd27a-9699-4585-9a35-1b57dbaefbbd, abstract = {{<p>Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its enzymatic hydrolysis was assessed by reducing sugar and high-performance anion-exchange chromatography analysis. The hydrolysis of the UFR preparation with Viscozyme L or <i>Trichoderma reesei</i> β-mannanase <i>Tr</i>Man5A (1 mg/mL) resulted in less than ~50% bioconversion of AcGGM. The AcGGM in the AD preparation was hydrolyzed to a higher degree (~67% with TrMan5A and 80% with Viscozyme L) and showed the highest conversion rate. This indicates that SSL contains enzyme-inhibitory compounds (e.g., lignin and lignin-derived compounds such as lignosulfonates) which were successfully removed. </p>}}, author = {{Bhattacharya, Abhishek and Butler, Samuel and Al-Rudainy, Basel and Wallberg, Ola and Stålbrand, Henrik}}, issn = {{1420-3049}}, keywords = {{Hydrolysis; Lignin/chemistry; Polysaccharides; Sulfites}}, language = {{eng}}, month = {{05}}, number = {{10}}, publisher = {{MDPI AG}}, series = {{Molecules (Basel, Switzerland)}}, title = {{Enzymatic Conversion of Different Qualities of Refined Softwood Hemicellulose Recovered from Spent Sulfite Liquor}}, url = {{http://dx.doi.org/10.3390/molecules27103207}}, doi = {{10.3390/molecules27103207}}, volume = {{27}}, year = {{2022}}, }