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Enzymatic Conversion of Different Qualities of Refined Softwood Hemicellulose Recovered from Spent Sulfite Liquor

Bhattacharya, Abhishek LU ; Butler, Samuel LU ; Al-Rudainy, Basel LU ; Wallberg, Ola LU orcid and Stålbrand, Henrik LU (2022) In Molecules (Basel, Switzerland) 27(10).
Abstract

Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its... (More)

Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its enzymatic hydrolysis was assessed by reducing sugar and high-performance anion-exchange chromatography analysis. The hydrolysis of the UFR preparation with Viscozyme L or Trichoderma reesei β-mannanase TrMan5A (1 mg/mL) resulted in less than ~50% bioconversion of AcGGM. The AcGGM in the AD preparation was hydrolyzed to a higher degree (~67% with TrMan5A and 80% with Viscozyme L) and showed the highest conversion rate. This indicates that SSL contains enzyme-inhibitory compounds (e.g., lignin and lignin-derived compounds such as lignosulfonates) which were successfully removed.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hydrolysis, Lignin/chemistry, Polysaccharides, Sulfites
in
Molecules (Basel, Switzerland)
volume
27
issue
10
article number
3207
pages
20 pages
publisher
MDPI AG
external identifiers
  • scopus:85131659678
  • pmid:35630684
ISSN
1420-3049
DOI
10.3390/molecules27103207
language
English
LU publication?
yes
id
334fd27a-9699-4585-9a35-1b57dbaefbbd
date added to LUP
2022-06-18 11:09:28
date last changed
2024-06-13 17:42:47
@article{334fd27a-9699-4585-9a35-1b57dbaefbbd,
  abstract     = {{<p>Spent sulfite liquor (SSL) from softwood processing is rich in hemicellulose (acetyl galactoglucomannan, AcGGM), lignin, and lignin-derived compounds. We investigated the effect of sequential AcGGM purification on the enzymatic bioconversion of AcGGM. SSL was processed through three consecutive purification steps (membrane filtration, precipitation, and adsorption) to obtain AcGGM with increasing purity. Significant reduction (~99%) in lignin content and modest loss (~18%) of polysaccharides was observed during purification from the least pure preparation (UFR), obtained by membrane filtration, compared to the purest preparation (AD), obtained by adsorption. AcGGM (~14.5 kDa) was the major polysaccharide in the preparations; its enzymatic hydrolysis was assessed by reducing sugar and high-performance anion-exchange chromatography analysis. The hydrolysis of the UFR preparation with Viscozyme L or <i>Trichoderma reesei</i> β-mannanase  <i>Tr</i>Man5A (1 mg/mL) resulted in less than ~50% bioconversion of AcGGM. The AcGGM in the AD preparation was hydrolyzed to a higher degree (~67% with  TrMan5A and 80% with Viscozyme L) and showed the highest conversion rate. This indicates that SSL contains enzyme-inhibitory compounds (e.g., lignin and lignin-derived compounds such as lignosulfonates) which were successfully removed. </p>}},
  author       = {{Bhattacharya, Abhishek and Butler, Samuel and Al-Rudainy, Basel and Wallberg, Ola and Stålbrand, Henrik}},
  issn         = {{1420-3049}},
  keywords     = {{Hydrolysis; Lignin/chemistry; Polysaccharides; Sulfites}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{10}},
  publisher    = {{MDPI AG}},
  series       = {{Molecules (Basel, Switzerland)}},
  title        = {{Enzymatic Conversion of Different Qualities of Refined Softwood Hemicellulose Recovered from Spent Sulfite Liquor}},
  url          = {{http://dx.doi.org/10.3390/molecules27103207}},
  doi          = {{10.3390/molecules27103207}},
  volume       = {{27}},
  year         = {{2022}},
}