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Tenascins are associated with lipid rafts isolated from mouse brain

Kappler, J; Baader, SL; Franken, S; Pesheva, P; Schilling, K; Rauch, Uwe LU and Gieselmann, V (2002) In Biochemical and Biophysical Research Communications 294(3). p.742-747
Abstract
Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain... (More)
Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts. (C) 2002 Elsevier Science (USA). All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cerebellum, tenascin, lipid rafts
in
Biochemical and Biophysical Research Communications
volume
294
issue
3
pages
742 - 747
publisher
Elsevier
external identifiers
  • wos:000176364700038
  • pmid:12056833
  • scopus:0036295721
ISSN
1090-2104
DOI
10.1016/S0006-291X(02)00520-X
language
English
LU publication?
yes
id
6b105584-7984-4dd9-bd06-95c3a471e99a (old id 335286)
date added to LUP
2007-10-29 14:29:30
date last changed
2017-01-01 06:46:03
@article{6b105584-7984-4dd9-bd06-95c3a471e99a,
  abstract     = {Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts. (C) 2002 Elsevier Science (USA). All rights reserved.},
  author       = {Kappler, J and Baader, SL and Franken, S and Pesheva, P and Schilling, K and Rauch, Uwe and Gieselmann, V},
  issn         = {1090-2104},
  keyword      = {cerebellum,tenascin,lipid rafts},
  language     = {eng},
  number       = {3},
  pages        = {742--747},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Tenascins are associated with lipid rafts isolated from mouse brain},
  url          = {http://dx.doi.org/10.1016/S0006-291X(02)00520-X},
  volume       = {294},
  year         = {2002},
}