Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli
(2002) In Biotechnology Progress 18(3). p.652-656- Abstract
- In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed... (More)
- In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed that cells expressing double VHb reached their maximal capacity of protein synthesis later during cultivation than cells expressing native VHb, and furthermore they reached considerably higher levels of ribosome and tRNA compared to that of the VHb-expressing cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/335584
- author
- Roos, Viktoria LU ; Andersson, C ; Arfvidsson, Cecilia LU ; Wahlund, Karl-Gustav LU and Bülow, Leif LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Progress
- volume
- 18
- issue
- 3
- pages
- 652 - 656
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000176178300036
- scopus:0035988089
- ISSN
- 1520-6033
- DOI
- 10.1021/bp020005v
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Pure and Applied Biochemistry (LTH) (011001005)
- id
- b7f288ef-a6df-41f6-966a-cbe5011ab0fd (old id 335584)
- date added to LUP
- 2016-04-01 17:06:01
- date last changed
- 2022-03-30 20:31:15
@article{b7f288ef-a6df-41f6-966a-cbe5011ab0fd, abstract = {{In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed that cells expressing double VHb reached their maximal capacity of protein synthesis later during cultivation than cells expressing native VHb, and furthermore they reached considerably higher levels of ribosome and tRNA compared to that of the VHb-expressing cells.}}, author = {{Roos, Viktoria and Andersson, C and Arfvidsson, Cecilia and Wahlund, Karl-Gustav and Bülow, Leif}}, issn = {{1520-6033}}, language = {{eng}}, number = {{3}}, pages = {{652--656}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biotechnology Progress}}, title = {{Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli}}, url = {{http://dx.doi.org/10.1021/bp020005v}}, doi = {{10.1021/bp020005v}}, volume = {{18}}, year = {{2002}}, }