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Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli

Roos, Viktoria LU ; Andersson, C ; Arfvidsson, Cecilia LU ; Wahlund, Karl-Gustav LU and Bülow, Leif LU (2002) In Biotechnology Progress 18(3). p.652-656
Abstract
In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed... (More)
In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed that cells expressing double VHb reached their maximal capacity of protein synthesis later during cultivation than cells expressing native VHb, and furthermore they reached considerably higher levels of ribosome and tRNA compared to that of the VHb-expressing cells. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
18
issue
3
pages
652 - 656
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000176178300036
  • scopus:0035988089
ISSN
1520-6033
DOI
10.1021/bp020005v
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Pure and Applied Biochemistry (LTH) (011001005)
id
b7f288ef-a6df-41f6-966a-cbe5011ab0fd (old id 335584)
date added to LUP
2016-04-01 17:06:01
date last changed
2020-01-12 20:03:09
@article{b7f288ef-a6df-41f6-966a-cbe5011ab0fd,
  abstract     = {In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia cola (double VHb). Escherichia cola cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed that cells expressing double VHb reached their maximal capacity of protein synthesis later during cultivation than cells expressing native VHb, and furthermore they reached considerably higher levels of ribosome and tRNA compared to that of the VHb-expressing cells.},
  author       = {Roos, Viktoria and Andersson, C and Arfvidsson, Cecilia and Wahlund, Karl-Gustav and Bülow, Leif},
  issn         = {1520-6033},
  language     = {eng},
  number       = {3},
  pages        = {652--656},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Biotechnology Progress},
  title        = {Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli},
  url          = {http://dx.doi.org/10.1021/bp020005v},
  doi          = {10.1021/bp020005v},
  volume       = {18},
  year         = {2002},
}