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Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells

Liu, Jian-Jun LU ; Wang, Junyang LU ; Hertervig, E; Nilsson, A and Duan, Rui-Dong LU (2002) In Anticancer Research 22(1A). p.263-266
Abstract
Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an... (More)
Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sphingomyelinase, caspase-3, proliferation, sulindac, apoptosis
in
Anticancer Research
volume
22
issue
1A
pages
263 - 266
publisher
International Institute of Cancer Research
external identifiers
  • wos:000175351900043
  • scopus:0036244224
ISSN
1791-7530
language
English
LU publication?
yes
id
127dbfbe-5479-46e5-9f82-6fb3ecb03a58 (old id 338731)
date added to LUP
2007-11-02 09:21:27
date last changed
2017-03-12 03:38:53
@article{127dbfbe-5479-46e5-9f82-6fb3ecb03a58,
  abstract     = {Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism.},
  author       = {Liu, Jian-Jun and Wang, Junyang and Hertervig, E and Nilsson, A and Duan, Rui-Dong},
  issn         = {1791-7530},
  keyword      = {sphingomyelinase,caspase-3,proliferation,sulindac,apoptosis},
  language     = {eng},
  number       = {1A},
  pages        = {263--266},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer Research},
  title        = {Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells},
  volume       = {22},
  year         = {2002},
}