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Improved chip design for integrated solid-phase microextraction in on-line proteomic sample preparation

Bergkvist, Jonas LU ; Ekström, Simon LU ; Wallman, Lars LU ; Löfgren, Mikael; Marko-Varga, György LU ; Nilsson, Johan LU and Laurell, Thomas LU (2002) In Proteomics 2(4). p.422-429
Abstract
A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the micro-fluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS(R) software package. The... (More)
A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the micro-fluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS(R) software package. The modeling and analysis of the previously reported weir-SMEC design indicates some severe drawbacks, that can be reduced by changing the microextraction chip geometry to the grid-SMEC design. The overall analytical performance was thereby improved and also verified by experimental work. Matrix-assisted laser desorption/ionization mass spectra of model peptides extracted from both the weir-SMEC and the new grid-SMEC support the numerical analysis results. Further use of numerical modeling and analysis of the SMEC structures is also discussed and suggested in this work. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sample preparation, solid phase, fluid dynamics
in
Proteomics
volume
2
issue
4
pages
422 - 429
publisher
John Wiley & Sons
external identifiers
  • pmid:12164701
  • wos:000175237800010
  • scopus:0036228540
ISSN
1615-9861
DOI
10.1002/1615-9861(200204)2:4<422::AID-PROT422>3.0.CO;2-1
language
English
LU publication?
yes
id
d1ec68c3-f264-43e5-b6ad-86b9fd0fac5f (old id 338807)
date added to LUP
2007-08-08 14:26:15
date last changed
2017-01-01 04:22:29
@article{d1ec68c3-f264-43e5-b6ad-86b9fd0fac5f,
  abstract     = {A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the micro-fluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS(R) software package. The modeling and analysis of the previously reported weir-SMEC design indicates some severe drawbacks, that can be reduced by changing the microextraction chip geometry to the grid-SMEC design. The overall analytical performance was thereby improved and also verified by experimental work. Matrix-assisted laser desorption/ionization mass spectra of model peptides extracted from both the weir-SMEC and the new grid-SMEC support the numerical analysis results. Further use of numerical modeling and analysis of the SMEC structures is also discussed and suggested in this work.},
  author       = {Bergkvist, Jonas and Ekström, Simon and Wallman, Lars and Löfgren, Mikael and Marko-Varga, György and Nilsson, Johan and Laurell, Thomas},
  issn         = {1615-9861},
  keyword      = {sample preparation,solid phase,fluid dynamics},
  language     = {eng},
  number       = {4},
  pages        = {422--429},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics},
  title        = {Improved chip design for integrated solid-phase microextraction in on-line proteomic sample preparation},
  url          = {http://dx.doi.org/10.1002/1615-9861(200204)2:4<422::AID-PROT422>3.0.CO;2-1},
  volume       = {2},
  year         = {2002},
}