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Gold nanoparticles explore cells : cellular uptake and their use as intracellular probes

Huefner, Anna; Septiadi, Dedy; Wilts, Bodo D; Patel, Imran I; Kuan, Wei-Li; Fragniere, Alexandra; Barker, Roger A LU and Mahajan, Sumeet (2014) In Methods 68(2). p.63-354
Abstract

Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell... (More)

Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Cell Tracking, Cytoplasm, Endocytosis, Gold, Humans, Imaging, Three-Dimensional, Metal Nanoparticles, Spectrum Analysis, Raman, Journal Article
in
Methods
volume
68
issue
2
pages
63 - 354
publisher
Elsevier
external identifiers
  • scopus:84902329592
ISSN
1095-9130
DOI
10.1016/j.ymeth.2014.02.006
language
English
LU publication?
no
id
33d24491-a2fd-47a6-9864-bf8ae3ac9fde
date added to LUP
2016-11-24 15:14:04
date last changed
2017-10-01 05:26:25
@article{33d24491-a2fd-47a6-9864-bf8ae3ac9fde,
  abstract     = {<p>Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.</p>},
  author       = {Huefner, Anna and Septiadi, Dedy and Wilts, Bodo D and Patel, Imran I and Kuan, Wei-Li and Fragniere, Alexandra and Barker, Roger A and Mahajan, Sumeet},
  issn         = {1095-9130},
  keyword      = {Cell Tracking,Cytoplasm,Endocytosis,Gold,Humans,Imaging, Three-Dimensional,Metal Nanoparticles,Spectrum Analysis, Raman,Journal Article},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {63--354},
  publisher    = {Elsevier},
  series       = {Methods},
  title        = {Gold nanoparticles explore cells : cellular uptake and their use as intracellular probes},
  url          = {http://dx.doi.org/10.1016/j.ymeth.2014.02.006},
  volume       = {68},
  year         = {2014},
}