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Targeted proteomics of hip articular cartilage in OA and fracture patients

Hosseininia, Shahrzad LU ; Önnerfjord, Patrik LU and Dahlberg, Leif E. LU (2018) In Journal of Orthopaedic Research
Abstract

Osteoarthritis (OA) is a common chronic disease, causing joint pain and reduced physical function. OA progresses slowly over a period of several years; to avoid an exacerbation of symptoms, it is critical to able to diagnose the disease as early as possible. The identification of disease-specific biomarkers may enable such an early diagnosis. The aim of this study was to investigate potential biomarkers of cartilage metabolism in OA using a targeted multiplex approach by single reaction monitoring. Intact looking cartilage of femoral heads from patients with OA (n = 9) or femoral neck fractures (n = 12) was examined. Variations and relative quantifications of 35 selected extracellular matrix (ECM) proteins were analyzed using nano-LC... (More)

Osteoarthritis (OA) is a common chronic disease, causing joint pain and reduced physical function. OA progresses slowly over a period of several years; to avoid an exacerbation of symptoms, it is critical to able to diagnose the disease as early as possible. The identification of disease-specific biomarkers may enable such an early diagnosis. The aim of this study was to investigate potential biomarkers of cartilage metabolism in OA using a targeted multiplex approach by single reaction monitoring. Intact looking cartilage of femoral heads from patients with OA (n = 9) or femoral neck fractures (n = 12) was examined. Variations and relative quantifications of 35 selected extracellular matrix (ECM) proteins were analyzed using nano-LC coupled to tandem mass spectrometry. Our study showed statistically significantly increased levels of asporin (ASPN), mimecan (MIME), matrilin-3 (MATN3), cartilage intermediate layer protein 2 (CILP-2), collagen VI, collagen II, and collagen III N-propeptide in OA cartilage compared with non-OA cartilage. The other proteins in the protein panel did not appear to be different between the two groups. In conclusion, we identified a number of cartilage matrix proteins which may represent early molecular changes in the OA process and may have potential to predict the development of OA.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
extracellular matrix, hip osteoarthritis, mass spectrometry, proteomics
in
Journal of Orthopaedic Research
publisher
John Wiley & Sons
external identifiers
  • scopus:85059556122
ISSN
0736-0266
DOI
10.1002/jor.24158
language
English
LU publication?
yes
id
33f31536-bcde-44de-8d68-1796e6956fcb
date added to LUP
2019-01-21 13:49:56
date last changed
2019-02-20 11:43:46
@article{33f31536-bcde-44de-8d68-1796e6956fcb,
  abstract     = {<p>Osteoarthritis (OA) is a common chronic disease, causing joint pain and reduced physical function. OA progresses slowly over a period of several years; to avoid an exacerbation of symptoms, it is critical to able to diagnose the disease as early as possible. The identification of disease-specific biomarkers may enable such an early diagnosis. The aim of this study was to investigate potential biomarkers of cartilage metabolism in OA using a targeted multiplex approach by single reaction monitoring. Intact looking cartilage of femoral heads from patients with OA (n = 9) or femoral neck fractures (n = 12) was examined. Variations and relative quantifications of 35 selected extracellular matrix (ECM) proteins were analyzed using nano-LC coupled to tandem mass spectrometry. Our study showed statistically significantly increased levels of asporin (ASPN), mimecan (MIME), matrilin-3 (MATN3), cartilage intermediate layer protein 2 (CILP-2), collagen VI, collagen II, and collagen III N-propeptide in OA cartilage compared with non-OA cartilage. The other proteins in the protein panel did not appear to be different between the two groups. In conclusion, we identified a number of cartilage matrix proteins which may represent early molecular changes in the OA process and may have potential to predict the development of OA.</p>},
  author       = {Hosseininia, Shahrzad and Önnerfjord, Patrik and Dahlberg, Leif E.},
  issn         = {0736-0266},
  keyword      = {extracellular matrix,hip osteoarthritis,mass spectrometry,proteomics},
  language     = {eng},
  month        = {10},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Orthopaedic Research},
  title        = {Targeted proteomics of hip articular cartilage in OA and fracture patients},
  url          = {http://dx.doi.org/10.1002/jor.24158},
  year         = {2018},
}