Advanced

Mechanism of murine V gamma 1(+) gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection

Matsuzaki, G; Yamada, Hisakata LU ; Kishihara, K; Yoshikai, Y and Nomoto, K (2002) In European Journal of Immunology 32(4). p.928-935
Abstract
Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response,... (More)
Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1(+) gammadelta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1(+) gammadelta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alphabeta T cells lack IL-12R expression. All the results suggest that the Vgamma1(+) gammadelta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gamma delta T cell, Listeria monocytogenes, IL-12, IFN-gamma
in
European Journal of Immunology
volume
32
issue
4
pages
928 - 935
publisher
John Wiley & Sons
external identifiers
  • pmid:11920558
  • wos:000174968700002
  • scopus:0036010964
ISSN
1521-4141
DOI
10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I
language
English
LU publication?
yes
id
6b116b49-6ae0-49ab-acfb-a673d262e16d (old id 340360)
date added to LUP
2007-11-19 14:13:12
date last changed
2017-04-23 03:29:18
@article{6b116b49-6ae0-49ab-acfb-a673d262e16d,
  abstract     = {Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1(+) gammadelta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1(+) gammadelta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alphabeta T cells lack IL-12R expression. All the results suggest that the Vgamma1(+) gammadelta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.},
  author       = {Matsuzaki, G and Yamada, Hisakata and Kishihara, K and Yoshikai, Y and Nomoto, K},
  issn         = {1521-4141},
  keyword      = {gamma delta T cell,Listeria monocytogenes,IL-12,IFN-gamma},
  language     = {eng},
  number       = {4},
  pages        = {928--935},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Mechanism of murine V gamma 1(+) gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection},
  url          = {http://dx.doi.org/10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I},
  volume       = {32},
  year         = {2002},
}