Advanced

Parkinson-like neurodegeneration induced by targeted overexpression of alpha-synuclein in the nigrostriatal system

Kirik, Deniz LU ; Rosenblad, C; Burer, C; Lundberg, Cecilia LU ; Johansen, TE; Muzyczka, N; Mandel, RJ and Björklund, Anders LU (2002) In Journal of Neuroscience 22(7). p.2780-2791
Abstract
Recombinant adeno-associated viral vectors display efficient tropism for transduction of the dopamine neurons of the substantia nigra. Taking advantage of this unique property of recombinant adeno-associated viral vectors, we expressed wildtype and A53T mutated human alpha-synuclein in the nigrostriatal dopamine neurons of adult rats for up to 6 months. Cellular and axonal pathology, including alpha-synuclein-positive cytoplasmic inclusions and swollen, dystrophic neurites similar to those seen in brains from patients with Parkinson's disease, developed progressively over time. These pathological alterations occurred preferentially in the nigral dopamine neurons and were not observed in other nondopaminergic neurons transduced by the same... (More)
Recombinant adeno-associated viral vectors display efficient tropism for transduction of the dopamine neurons of the substantia nigra. Taking advantage of this unique property of recombinant adeno-associated viral vectors, we expressed wildtype and A53T mutated human alpha-synuclein in the nigrostriatal dopamine neurons of adult rats for up to 6 months. Cellular and axonal pathology, including alpha-synuclein-positive cytoplasmic inclusions and swollen, dystrophic neurites similar to those seen in brains from patients with Parkinson's disease, developed progressively over time. These pathological alterations occurred preferentially in the nigral dopamine neurons and were not observed in other nondopaminergic neurons transduced by the same vectors. The degenerative changes were accompanied by a loss of 30-80% of the nigral dopamine neurons, a 40-50% reduction of striatal dopamine, and tyrosine hydroxylase levels that was fully developed by 8 weeks. Significant motor impairment developed in those animals in which dopamine neuron cell loss exceeded a critical threshold of 50-60%. At 6 months, signs of cell body and axonal pathology had subsided, suggesting that the surviving neurons had recovered from the initial insult, despite the fact that alpha-synuclein expression was maintained at a high level. These results show that nigral dopamine neurons are selectively vulnerable to high levels of either wild-type or mutant alpha-synuclein, pointing to a key role for alpha-synuclein in the pathogenesis of Parkinson's disease. Targeted overexpression of alpha-synuclein in the nigrostriatal system may provide a new animal model of Parkinson's disease that reproduces some of the cardinal pathological, neurochemical, and behavioral features of the human disease. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tyrosine hydroxylase, nigral inclusion, dopamine, neurodegeneration, Parkinson's disease, adeno-associated virus vector
in
Journal of Neuroscience
volume
22
issue
7
pages
2780 - 2791
publisher
Society for Neuroscience
external identifiers
  • pmid:11923443
  • wos:000174626000041
ISSN
1529-2401
language
English
LU publication?
yes
id
e7a039a0-0c36-4e6e-89b9-10c735eba3f7 (old id 341055)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11923443&dopt=Abstract
http://www.jneurosci.org/cgi/content/abstract/22/7/2780
date added to LUP
2007-10-31 09:11:11
date last changed
2016-04-15 18:43:41
@article{e7a039a0-0c36-4e6e-89b9-10c735eba3f7,
  abstract     = {Recombinant adeno-associated viral vectors display efficient tropism for transduction of the dopamine neurons of the substantia nigra. Taking advantage of this unique property of recombinant adeno-associated viral vectors, we expressed wildtype and A53T mutated human alpha-synuclein in the nigrostriatal dopamine neurons of adult rats for up to 6 months. Cellular and axonal pathology, including alpha-synuclein-positive cytoplasmic inclusions and swollen, dystrophic neurites similar to those seen in brains from patients with Parkinson's disease, developed progressively over time. These pathological alterations occurred preferentially in the nigral dopamine neurons and were not observed in other nondopaminergic neurons transduced by the same vectors. The degenerative changes were accompanied by a loss of 30-80% of the nigral dopamine neurons, a 40-50% reduction of striatal dopamine, and tyrosine hydroxylase levels that was fully developed by 8 weeks. Significant motor impairment developed in those animals in which dopamine neuron cell loss exceeded a critical threshold of 50-60%. At 6 months, signs of cell body and axonal pathology had subsided, suggesting that the surviving neurons had recovered from the initial insult, despite the fact that alpha-synuclein expression was maintained at a high level. These results show that nigral dopamine neurons are selectively vulnerable to high levels of either wild-type or mutant alpha-synuclein, pointing to a key role for alpha-synuclein in the pathogenesis of Parkinson's disease. Targeted overexpression of alpha-synuclein in the nigrostriatal system may provide a new animal model of Parkinson's disease that reproduces some of the cardinal pathological, neurochemical, and behavioral features of the human disease.},
  author       = {Kirik, Deniz and Rosenblad, C and Burer, C and Lundberg, Cecilia and Johansen, TE and Muzyczka, N and Mandel, RJ and Björklund, Anders},
  issn         = {1529-2401},
  keyword      = {tyrosine hydroxylase,nigral inclusion,dopamine,neurodegeneration,Parkinson's disease,adeno-associated virus vector},
  language     = {eng},
  number       = {7},
  pages        = {2780--2791},
  publisher    = {Society for Neuroscience},
  series       = {Journal of Neuroscience},
  title        = {Parkinson-like neurodegeneration induced by targeted overexpression of alpha-synuclein in the nigrostriatal system},
  volume       = {22},
  year         = {2002},
}