Advanced

Influence of prior Cd2+ exposure on the uptake of Cd2+ and other elements in the phytochelatin-deficient mutant, cad1-3, of Arabidopsis thaliana

Larsson, E Helene; Asp, H and Bornman, Janet LU (2002) In Journal of Experimental Botany 53(368). p.447-453
Abstract
In order to test the potential effect of prior exposure to different Cd concentrations on Cd uptake and accumulation, plants of Arabidopsis thaliana, including a phytochelatin-deficient mutant, cad1-3, and the wild type, were compared. For Cd uptake experiments, plants were grown for 1 week in nutrient solution containing different Cd concentrations (0, 0.05, 0.1, 0.25, 0.5, and 1.0 muM Cd(NO3)(2)). Thereafter they were subjected to 0.5 muM Cd labelled with Cd-109 for 2 h. Uptake experiments with Cd-109 showed that the phytochelatin-deficient mutant cad1-3, accumulated less Cd than the wild type. Both a lower proportion and lower total amount of absorbed Cd were translocated to the shoot in cad1-3 plants compared to wild-type plants.... (More)
In order to test the potential effect of prior exposure to different Cd concentrations on Cd uptake and accumulation, plants of Arabidopsis thaliana, including a phytochelatin-deficient mutant, cad1-3, and the wild type, were compared. For Cd uptake experiments, plants were grown for 1 week in nutrient solution containing different Cd concentrations (0, 0.05, 0.1, 0.25, 0.5, and 1.0 muM Cd(NO3)(2)). Thereafter they were subjected to 0.5 muM Cd labelled with Cd-109 for 2 h. Uptake experiments with Cd-109 showed that the phytochelatin-deficient mutant cad1-3, accumulated less Cd than the wild type. Both a lower proportion and lower total amount of absorbed Cd were translocated to the shoot in cad1-3 plants compared to wild-type plants. Cadmium exposure also influenced the amounts of nutrients found, whereby after exposure to high Cd concentrations (0.5, 1.0 muM) during growth, cad1-3 roots contained less Fe, K, Mg, P, and S compared to roots of the wild type. In cad1-3 these elements decreased with increasing Cd concentration. The total Cd content in roots and shoots increased significantly with increasing Cd concentration during growth, although the increase was much less in cad1-3 plants. In time-dependent experiments of Cd uptake carried out between 15 and 120 min on plants not previously exposed to Cd, no significant difference in Cd accumulation between the mutant and wild type were found, although a smaller amount of Cd was translocated to the shoot in cad1-3 plants. The possibility that the differences in Cd accumulation in mutant and wild-type lines may be due to the cytosolic Cd regulation, which is inhibited by the complexation of Cd by phytochelatins, is discussed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cadmium, Arabidopsis thaliana, cad1-3
in
Journal of Experimental Botany
volume
53
issue
368
pages
447 - 453
publisher
Oxford University Press
external identifiers
  • wos:000174077200008
  • pmid:11847243
  • scopus:0036008861
ISSN
0022-0957
language
English
LU publication?
yes
id
18587e46-828f-4762-8d41-1cd8cea9213e (old id 342857)
date added to LUP
2007-11-07 16:18:16
date last changed
2017-11-05 03:43:17
@article{18587e46-828f-4762-8d41-1cd8cea9213e,
  abstract     = {In order to test the potential effect of prior exposure to different Cd concentrations on Cd uptake and accumulation, plants of Arabidopsis thaliana, including a phytochelatin-deficient mutant, cad1-3, and the wild type, were compared. For Cd uptake experiments, plants were grown for 1 week in nutrient solution containing different Cd concentrations (0, 0.05, 0.1, 0.25, 0.5, and 1.0 muM Cd(NO3)(2)). Thereafter they were subjected to 0.5 muM Cd labelled with Cd-109 for 2 h. Uptake experiments with Cd-109 showed that the phytochelatin-deficient mutant cad1-3, accumulated less Cd than the wild type. Both a lower proportion and lower total amount of absorbed Cd were translocated to the shoot in cad1-3 plants compared to wild-type plants. Cadmium exposure also influenced the amounts of nutrients found, whereby after exposure to high Cd concentrations (0.5, 1.0 muM) during growth, cad1-3 roots contained less Fe, K, Mg, P, and S compared to roots of the wild type. In cad1-3 these elements decreased with increasing Cd concentration. The total Cd content in roots and shoots increased significantly with increasing Cd concentration during growth, although the increase was much less in cad1-3 plants. In time-dependent experiments of Cd uptake carried out between 15 and 120 min on plants not previously exposed to Cd, no significant difference in Cd accumulation between the mutant and wild type were found, although a smaller amount of Cd was translocated to the shoot in cad1-3 plants. The possibility that the differences in Cd accumulation in mutant and wild-type lines may be due to the cytosolic Cd regulation, which is inhibited by the complexation of Cd by phytochelatins, is discussed.},
  author       = {Larsson, E Helene and Asp, H and Bornman, Janet},
  issn         = {0022-0957},
  keyword      = {cadmium,Arabidopsis thaliana,cad1-3},
  language     = {eng},
  number       = {368},
  pages        = {447--453},
  publisher    = {Oxford University Press},
  series       = {Journal of Experimental Botany},
  title        = {Influence of prior Cd2+ exposure on the uptake of Cd2+ and other elements in the phytochelatin-deficient mutant, cad1-3, of Arabidopsis thaliana},
  volume       = {53},
  year         = {2002},
}