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Characterization of a novel testicular form of human hormone-sensitive lipase

Mairal, A; Melaine, N; Laurell, H; Grober, J; Stenson, Lena LU ; Guillaudeux, T; Holm, Cecilia LU ; Jegou, B and Langin, D (2002) In Biochemical and Biophysical Research Communications 291(2). p.286-290
Abstract
Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
testis, spermatogenesis, lipid metabolism, fertility, lipase, esterase
in
Biochemical and Biophysical Research Communications
volume
291
issue
2
pages
286 - 290
publisher
Elsevier
external identifiers
  • wos:000173990800014
  • pmid:11846402
  • scopus:0036289445
ISSN
1090-2104
DOI
10.1006/bbrc.2002.6427
language
English
LU publication?
yes
id
942b5406-b841-4ead-af5d-d9f1521b1bb1 (old id 342994)
date added to LUP
2007-11-02 10:49:08
date last changed
2017-08-06 04:34:25
@article{942b5406-b841-4ead-af5d-d9f1521b1bb1,
  abstract     = {Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.},
  author       = {Mairal, A and Melaine, N and Laurell, H and Grober, J and Stenson, Lena and Guillaudeux, T and Holm, Cecilia and Jegou, B and Langin, D},
  issn         = {1090-2104},
  keyword      = {testis,spermatogenesis,lipid metabolism,fertility,lipase,esterase},
  language     = {eng},
  number       = {2},
  pages        = {286--290},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Characterization of a novel testicular form of human hormone-sensitive lipase},
  url          = {http://dx.doi.org/10.1006/bbrc.2002.6427},
  volume       = {291},
  year         = {2002},
}