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Expression of MUC5AC and MUC5B mucins in normal and cystic fibrosis lung

Groneberg, DA; Eynott, PR; Oates, T; Lim, S; Wu, R; Carlstedt, Ingemar LU ; Nicholson, AG and Chung, KF (2002) In Respiratory Medicine 96(2). p.81-86
Abstract
Hypersecretion of airway mucus is a characteristic feature of chronic airway diseases like cystic fibrosis (CF) and leads via impairment of the muco-ciliary clearance and bacterial superinfection to respiratory failure. The major components of the mucus matrix forming family of mucins in the airways are MUC5AC and MUC5B. To investigate the expression of these glycoproteins in CF, immunohistochemistry was carried out on trachea, bronchi and peripheral lung obtained from CF patients and compared to normal lung tissues. MUC5AC immunohistochemistry demonstrated signals in goblet cells of the epithelial lining. Also, goblet cells inside glandular secretory ducts revealed MUC5AC-positive staining. In comparison to those from normal subjects, CF... (More)
Hypersecretion of airway mucus is a characteristic feature of chronic airway diseases like cystic fibrosis (CF) and leads via impairment of the muco-ciliary clearance and bacterial superinfection to respiratory failure. The major components of the mucus matrix forming family of mucins in the airways are MUC5AC and MUC5B. To investigate the expression of these glycoproteins in CF, immunohistochemistry was carried out on trachea, bronchi and peripheral lung obtained from CF patients and compared to normal lung tissues. MUC5AC immunohistochemistry demonstrated signals in goblet cells of the epithelial lining. Also, goblet cells inside glandular secretory ducts revealed MUC5AC-positive staining. In comparison to those from normal subjects, CF sections were characterized by inflammatory changes and goblet cell hyperplasia, resulting in increased numbers of MUC5AC-positive cells. Immunohistochemical staining for MUC5B showed abundant staining of submucosal glands and epithelial goblet cells. Inside the glands, the immunoreactivity was restricted to glandular mucous cells, MUC5AC and MUC5B are expressed in the same histological pattern in CF compared to normal tissues with an increase of MUC5AC-positive cells due to goblet cell hyper- and metaplasia. (C) 2001 Elsevier Science Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunohistochemistry, lung, mucin, cystic fibrosis
in
Respiratory Medicine
volume
96
issue
2
pages
81 - 86
publisher
Elsevier
external identifiers
  • wos:000173871300002
  • pmid:11860173
  • scopus:0036486435
ISSN
1532-3064
DOI
10.1053/rmed.2001.1221
language
English
LU publication?
yes
id
fa339217-0aeb-48b0-a928-580baf85f6ce (old id 343031)
date added to LUP
2007-08-21 16:05:07
date last changed
2017-11-12 03:53:03
@article{fa339217-0aeb-48b0-a928-580baf85f6ce,
  abstract     = {Hypersecretion of airway mucus is a characteristic feature of chronic airway diseases like cystic fibrosis (CF) and leads via impairment of the muco-ciliary clearance and bacterial superinfection to respiratory failure. The major components of the mucus matrix forming family of mucins in the airways are MUC5AC and MUC5B. To investigate the expression of these glycoproteins in CF, immunohistochemistry was carried out on trachea, bronchi and peripheral lung obtained from CF patients and compared to normal lung tissues. MUC5AC immunohistochemistry demonstrated signals in goblet cells of the epithelial lining. Also, goblet cells inside glandular secretory ducts revealed MUC5AC-positive staining. In comparison to those from normal subjects, CF sections were characterized by inflammatory changes and goblet cell hyperplasia, resulting in increased numbers of MUC5AC-positive cells. Immunohistochemical staining for MUC5B showed abundant staining of submucosal glands and epithelial goblet cells. Inside the glands, the immunoreactivity was restricted to glandular mucous cells, MUC5AC and MUC5B are expressed in the same histological pattern in CF compared to normal tissues with an increase of MUC5AC-positive cells due to goblet cell hyper- and metaplasia. (C) 2001 Elsevier Science Ltd.},
  author       = {Groneberg, DA and Eynott, PR and Oates, T and Lim, S and Wu, R and Carlstedt, Ingemar and Nicholson, AG and Chung, KF},
  issn         = {1532-3064},
  keyword      = {immunohistochemistry,lung,mucin,cystic fibrosis},
  language     = {eng},
  number       = {2},
  pages        = {81--86},
  publisher    = {Elsevier},
  series       = {Respiratory Medicine},
  title        = {Expression of MUC5AC and MUC5B mucins in normal and cystic fibrosis lung},
  url          = {http://dx.doi.org/10.1053/rmed.2001.1221},
  volume       = {96},
  year         = {2002},
}