Molecular mass distribution analysis of ethyl(hydroxyethyl)cellulose by size-exclusion chromatography with dual light-scattering and refractometric detection
(2002) In Journal of Chromatography A 946(1-2). p.69-81- Abstract
- Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations... (More)
- Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations of aggregate behavior as well as model size-exclusion experiments with latex particles indicate that they partly carry a minute charge and have a compact structure. Their weak retention under the separation conditions used suggests a difference in their surface chemistry when compared with the dissolved polymer coils which exhibit a correct size-exclusion behavior. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/343420
- author
- Porsch, B ; Andersson, Mats ; Wittgren, B and Wahlund, Karl-Gustav LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- detection, ethyl(hydroxyethyl)cellulose, LC
- in
- Journal of Chromatography A
- volume
- 946
- issue
- 1-2
- pages
- 69 - 81
- publisher
- Elsevier
- external identifiers
-
- pmid:11873984
- wos:000173820700007
- scopus:0037039723
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)01572-2
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- d9e06357-ab7a-46be-9041-bf6700eee954 (old id 343420)
- date added to LUP
- 2016-04-01 16:23:47
- date last changed
- 2022-01-28 19:23:15
@article{d9e06357-ab7a-46be-9041-bf6700eee954,
abstract = {{Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations of aggregate behavior as well as model size-exclusion experiments with latex particles indicate that they partly carry a minute charge and have a compact structure. Their weak retention under the separation conditions used suggests a difference in their surface chemistry when compared with the dissolved polymer coils which exhibit a correct size-exclusion behavior. (C) 2002 Elsevier Science B.V. All rights reserved.}},
author = {{Porsch, B and Andersson, Mats and Wittgren, B and Wahlund, Karl-Gustav}},
issn = {{0021-9673}},
keywords = {{detection; ethyl(hydroxyethyl)cellulose; LC}},
language = {{eng}},
number = {{1-2}},
pages = {{69--81}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Molecular mass distribution analysis of ethyl(hydroxyethyl)cellulose by size-exclusion chromatography with dual light-scattering and refractometric detection}},
url = {{http://dx.doi.org/10.1016/S0021-9673(01)01572-2}},
doi = {{10.1016/S0021-9673(01)01572-2}},
volume = {{946}},
year = {{2002}},
}