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Molecular mass distribution analysis of ethyl(hydroxyethyl)cellulose by size-exclusion chromatography with dual light-scattering and refractometric detection

Porsch, B; Andersson, Mats; Wittgren, B and Wahlund, Karl-Gustav LU (2002) In Journal of Chromatography A 946(1-2). p.69-81
Abstract
Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations... (More)
Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations of aggregate behavior as well as model size-exclusion experiments with latex particles indicate that they partly carry a minute charge and have a compact structure. Their weak retention under the separation conditions used suggests a difference in their surface chemistry when compared with the dissolved polymer coils which exhibit a correct size-exclusion behavior. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
detection, ethyl(hydroxyethyl)cellulose, LC
in
Journal of Chromatography A
volume
946
issue
1-2
pages
69 - 81
publisher
Elsevier
external identifiers
  • pmid:11873984
  • wos:000173820700007
  • scopus:0037039723
ISSN
0021-9673
DOI
10.1016/S0021-9673(01)01572-2
language
English
LU publication?
yes
id
d9e06357-ab7a-46be-9041-bf6700eee954 (old id 343420)
date added to LUP
2007-11-09 12:08:03
date last changed
2017-01-01 07:04:08
@article{d9e06357-ab7a-46be-9041-bf6700eee954,
  abstract     = {Dual low-angle light scattering and refractometric detection coupled to size-exclusion chromatography provided proof for the presence of a low amount of stable aggregates/particles in ethyl(hydroxyethyl)cellulose. Unlike the correct size-exclusion chromatographic behavior of the parent polysaccharide itself, the aggregates exhibit variable size-dependent weak retention as a function of flow-rate and of ionic strength of the aqueous mobile phase. Therefore, determination of the molecular mass of non-aggregated polymer is possible in aqueous mobile phase containing 0.1 M NaCl under conditions at which aggregates are completely adsorbed on the column packing irrespective of the flow-rate used. Flow-rate and ionic strength-dependent variations of aggregate behavior as well as model size-exclusion experiments with latex particles indicate that they partly carry a minute charge and have a compact structure. Their weak retention under the separation conditions used suggests a difference in their surface chemistry when compared with the dissolved polymer coils which exhibit a correct size-exclusion behavior. (C) 2002 Elsevier Science B.V. All rights reserved.},
  author       = {Porsch, B and Andersson, Mats and Wittgren, B and Wahlund, Karl-Gustav},
  issn         = {0021-9673},
  keyword      = {detection,ethyl(hydroxyethyl)cellulose,LC},
  language     = {eng},
  number       = {1-2},
  pages        = {69--81},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Molecular mass distribution analysis of ethyl(hydroxyethyl)cellulose by size-exclusion chromatography with dual light-scattering and refractometric detection},
  url          = {http://dx.doi.org/10.1016/S0021-9673(01)01572-2},
  volume       = {946},
  year         = {2002},
}