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Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult, mouse central nervous systems

Oohashi, T; Hirakawa, S; Bekku, Y; Rauch, Uwe LU ; Zimmermann, D R; Su, W D; Ohtsuka, A; Murakami, T and Ninomiya, Y (2002) In Molecular and Cellular Neuroscience 19(1). p.43-57
Abstract
Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform... (More)
Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform at the nodes of Ranvier. The present data suggest that Bral1 may play a pivotal role in the formation of the hyaluronan-associated matrix in the CNS that facilitates neuronal conduction by forming an ion diffusion barrier at the nodes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Neuroscience
volume
19
issue
1
pages
43 - 57
publisher
Elsevier
external identifiers
  • pmid:11817897
  • wos:000173605100004
  • scopus:0036152582
ISSN
1044-7431
DOI
10.1006/mcne.2001.1061
language
English
LU publication?
yes
id
b6289f99-38bf-4dfa-be9e-8d50703159fc (old id 343756)
date added to LUP
2007-11-02 14:29:22
date last changed
2017-10-29 03:38:35
@article{b6289f99-38bf-4dfa-be9e-8d50703159fc,
  abstract     = {Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform at the nodes of Ranvier. The present data suggest that Bral1 may play a pivotal role in the formation of the hyaluronan-associated matrix in the CNS that facilitates neuronal conduction by forming an ion diffusion barrier at the nodes.},
  author       = {Oohashi, T and Hirakawa, S and Bekku, Y and Rauch, Uwe and Zimmermann, D R and Su, W D and Ohtsuka, A and Murakami, T and Ninomiya, Y},
  issn         = {1044-7431},
  language     = {eng},
  number       = {1},
  pages        = {43--57},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult, mouse central nervous systems},
  url          = {http://dx.doi.org/10.1006/mcne.2001.1061},
  volume       = {19},
  year         = {2002},
}