Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult, mouse central nervous systems
(2002) In Molecular and Cellular Neuroscience 19(1). p.43-57- Abstract
- Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform... (More)
- Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform at the nodes of Ranvier. The present data suggest that Bral1 may play a pivotal role in the formation of the hyaluronan-associated matrix in the CNS that facilitates neuronal conduction by forming an ion diffusion barrier at the nodes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/343756
- author
- Oohashi, T ; Hirakawa, S ; Bekku, Y ; Rauch, Uwe LU ; Zimmermann, D R ; Su, W D ; Ohtsuka, A ; Murakami, T and Ninomiya, Y
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Neuroscience
- volume
- 19
- issue
- 1
- pages
- 43 - 57
- publisher
- Elsevier
- external identifiers
-
- pmid:11817897
- wos:000173605100004
- scopus:0036152582
- pmid:11817897
- ISSN
- 1044-7431
- DOI
- 10.1006/mcne.2001.1061
- language
- English
- LU publication?
- yes
- id
- b6289f99-38bf-4dfa-be9e-8d50703159fc (old id 343756)
- date added to LUP
- 2016-04-01 12:17:17
- date last changed
- 2022-04-13 08:55:02
@article{b6289f99-38bf-4dfa-be9e-8d50703159fc, abstract = {{Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform at the nodes of Ranvier. The present data suggest that Bral1 may play a pivotal role in the formation of the hyaluronan-associated matrix in the CNS that facilitates neuronal conduction by forming an ion diffusion barrier at the nodes.}}, author = {{Oohashi, T and Hirakawa, S and Bekku, Y and Rauch, Uwe and Zimmermann, D R and Su, W D and Ohtsuka, A and Murakami, T and Ninomiya, Y}}, issn = {{1044-7431}}, language = {{eng}}, number = {{1}}, pages = {{43--57}}, publisher = {{Elsevier}}, series = {{Molecular and Cellular Neuroscience}}, title = {{Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult, mouse central nervous systems}}, url = {{http://dx.doi.org/10.1006/mcne.2001.1061}}, doi = {{10.1006/mcne.2001.1061}}, volume = {{19}}, year = {{2002}}, }