Advanced

Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.

Kirkeby, Agnete LU ; Nelander Wahlestedt, Jenny LU and Parmar, Malin LU (2012) In Frontiers in Cellular Neuroscience 6(Published online: 03 January 2013).
Abstract
Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human... (More)
Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Cellular Neuroscience
volume
6
issue
Published online: 03 January 2013
publisher
Frontiers
external identifiers
  • wos:000312979800001
  • pmid:23316134
  • scopus:84872186092
ISSN
1662-5102
DOI
10.3389/fncel.2012.00064
language
English
LU publication?
yes
id
3b917c48-2515-456a-923c-02584939e74c (old id 3438755)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23316134?dopt=Abstract
date added to LUP
2013-02-04 12:50:48
date last changed
2017-06-18 04:02:39
@article{3b917c48-2515-456a-923c-02584939e74c,
  abstract     = {Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.},
  articleno    = {64},
  author       = {Kirkeby, Agnete and Nelander Wahlestedt, Jenny and Parmar, Malin},
  issn         = {1662-5102},
  language     = {eng},
  number       = {Published online: 03 January 2013},
  publisher    = {Frontiers},
  series       = {Frontiers in Cellular Neuroscience},
  title        = {Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.},
  url          = {http://dx.doi.org/10.3389/fncel.2012.00064},
  volume       = {6},
  year         = {2012},
}