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Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia

Ryding, Ulf LU ; Espersen, F; Soderquist, B and Christensson, B (2002) In Diagnostic Microbiology and Infectious Disease 42(1). p.9-15
Abstract
Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell... (More)
Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid, a-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia. (C) 2002 Elsevier Science Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diagnostic Microbiology and Infectious Disease
volume
42
issue
1
pages
9 - 15
publisher
Elsevier
external identifiers
  • pmid:11821165
  • wos:000173694900002
  • scopus:0036159479
ISSN
1879-0070
DOI
10.1016/S0732-8893(01)00311-X
language
English
LU publication?
yes
id
e9d30f1b-97a3-45cc-a1cd-f39ff713ba97 (old id 343920)
date added to LUP
2007-11-12 14:11:50
date last changed
2017-01-01 04:19:49
@article{e9d30f1b-97a3-45cc-a1cd-f39ff713ba97,
  abstract     = {Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid, a-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia. (C) 2002 Elsevier Science Inc. All rights reserved.},
  author       = {Ryding, Ulf and Espersen, F and Soderquist, B and Christensson, B},
  issn         = {1879-0070},
  language     = {eng},
  number       = {1},
  pages        = {9--15},
  publisher    = {Elsevier},
  series       = {Diagnostic Microbiology and Infectious Disease},
  title        = {Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia},
  url          = {http://dx.doi.org/10.1016/S0732-8893(01)00311-X},
  volume       = {42},
  year         = {2002},
}