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Identification of a group of nontuberculous mycobacteria isolated from the South Indian BCG trial area by HPLC

Vanitha, JD; Immanuel, C; Szponar, Bogumila LU ; Larsson, Lennart LU and Paramasivan, CN (2002) In Current Science 82(2). p.189-191
Abstract
Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing... (More)
Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing mycobacteria. The results show that HPLC can discriminate the described mycobacterial species better than biochemical methods and GC-MS. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Science
volume
82
issue
2
pages
189 - 191
publisher
Current Science
external identifiers
  • wos:000173671700024
  • scopus:0037905344
ISSN
0011-3891
language
English
LU publication?
yes
id
9c8eadfa-abb3-418b-bed5-e96cd83872de (old id 343949)
alternative location
http://www.ias.ac.in/currsci/jan252002/189.pdf
date added to LUP
2007-11-13 14:41:56
date last changed
2017-01-01 07:24:07
@article{9c8eadfa-abb3-418b-bed5-e96cd83872de,
  abstract     = {Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing mycobacteria. The results show that HPLC can discriminate the described mycobacterial species better than biochemical methods and GC-MS.},
  author       = {Vanitha, JD and Immanuel, C and Szponar, Bogumila and Larsson, Lennart and Paramasivan, CN},
  issn         = {0011-3891},
  language     = {eng},
  number       = {2},
  pages        = {189--191},
  publisher    = {Current Science},
  series       = {Current Science},
  title        = {Identification of a group of nontuberculous mycobacteria isolated from the South Indian BCG trial area by HPLC},
  volume       = {82},
  year         = {2002},
}