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Hepatic metabolism of propionate and methylmalonate in growing lambs

Berthelot, V; Pierzynowski, Stefan LU ; Sauvant, D and Kristensen, NB (2002) In Livestock Production Science1974-01-01+01:002005-01-01+01:00 74(1). p.33-43
Abstract
The hepatic extraction ratio or propionate and the net hepatic flux of methylmalonate (MMA) were investigated in six Suffolk lambs (32.7 +/- 1.7 kg BW) implanted with catheters in a mesenteric artery and in the portal, hepatic, and ruminal veins and a cannula in the rumen. The lambs were fed a pelleted barley-based diet (1.1 kg DM per day) and subjected to three intravenous infusion protocols separated by at least 7 days: saline (C; control), butyrate (B; 0.2 mmol/h/kg BW) or lactate (L; 0.96 mmol/h/kg BW). The solutions were infused continuously into the ruminal vein for 13 h. At the same time as the intravenous infusion treatments, propionate (1.78 mmol/h/kg BW) was infused intraruminally for 4 h starting 4 h after the initiation of the... (More)
The hepatic extraction ratio or propionate and the net hepatic flux of methylmalonate (MMA) were investigated in six Suffolk lambs (32.7 +/- 1.7 kg BW) implanted with catheters in a mesenteric artery and in the portal, hepatic, and ruminal veins and a cannula in the rumen. The lambs were fed a pelleted barley-based diet (1.1 kg DM per day) and subjected to three intravenous infusion protocols separated by at least 7 days: saline (C; control), butyrate (B; 0.2 mmol/h/kg BW) or lactate (L; 0.96 mmol/h/kg BW). The solutions were infused continuously into the ruminal vein for 13 h. At the same time as the intravenous infusion treatments, propionate (1.78 mmol/h/kg BW) was infused intraruminally for 4 h starting 4 h after the initiation of the experimental protocol. Arterial, portal and hepatic blood samples were obtained during the last 10 h of the infusion protocol. Blood flow was measured by down stream dilution of p-aminohippuric acid. The hepatic extraction ratio of propionate decreased from 85 +/- 3 to 75 +/- 1% after intraruminal infusion of propionate and the net splanchnic appearance of propionate increased threefold with the doubling of the net portal appearance of propionate. Intravenous infusion of lactate or butyrate did not affect the hepatic extraction ratio or net splanchnic flux of propionate. The net portal appearance, net hepatic flux and net splanchnic appearance of MMA were not (P > 0.10) different from zero in all situations. The arterial concentration of MMA increased with increasing absorption rate of propionate but remained below 2 mumol/l throughout the experiment. The results of the present study do not support the hypothesis that the MMA possibly incorporated into methyl-branched chain fatty acids in adipose tissue of intensively-reared lambs is of hepatic origin. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
branched chain fatty acids, methylmalonic acid, propionate, sheep, liver
in
Livestock Production Science1974-01-01+01:002005-01-01+01:00
volume
74
issue
1
pages
33 - 43
publisher
Elsevier
external identifiers
  • wos:000173423300004
  • scopus:0036161987
ISSN
0301-6226
DOI
10.1016/S0301-6226(01)00281-0
language
English
LU publication?
yes
id
b4c5eba7-3f2e-4b7c-871b-9c606368d818 (old id 344385)
date added to LUP
2007-08-10 08:40:19
date last changed
2017-12-05 10:40:18
@article{b4c5eba7-3f2e-4b7c-871b-9c606368d818,
  abstract     = {The hepatic extraction ratio or propionate and the net hepatic flux of methylmalonate (MMA) were investigated in six Suffolk lambs (32.7 +/- 1.7 kg BW) implanted with catheters in a mesenteric artery and in the portal, hepatic, and ruminal veins and a cannula in the rumen. The lambs were fed a pelleted barley-based diet (1.1 kg DM per day) and subjected to three intravenous infusion protocols separated by at least 7 days: saline (C; control), butyrate (B; 0.2 mmol/h/kg BW) or lactate (L; 0.96 mmol/h/kg BW). The solutions were infused continuously into the ruminal vein for 13 h. At the same time as the intravenous infusion treatments, propionate (1.78 mmol/h/kg BW) was infused intraruminally for 4 h starting 4 h after the initiation of the experimental protocol. Arterial, portal and hepatic blood samples were obtained during the last 10 h of the infusion protocol. Blood flow was measured by down stream dilution of p-aminohippuric acid. The hepatic extraction ratio of propionate decreased from 85 +/- 3 to 75 +/- 1% after intraruminal infusion of propionate and the net splanchnic appearance of propionate increased threefold with the doubling of the net portal appearance of propionate. Intravenous infusion of lactate or butyrate did not affect the hepatic extraction ratio or net splanchnic flux of propionate. The net portal appearance, net hepatic flux and net splanchnic appearance of MMA were not (P > 0.10) different from zero in all situations. The arterial concentration of MMA increased with increasing absorption rate of propionate but remained below 2 mumol/l throughout the experiment. The results of the present study do not support the hypothesis that the MMA possibly incorporated into methyl-branched chain fatty acids in adipose tissue of intensively-reared lambs is of hepatic origin.},
  author       = {Berthelot, V and Pierzynowski, Stefan and Sauvant, D and Kristensen, NB},
  issn         = {0301-6226},
  keyword      = {branched chain fatty acids,methylmalonic acid,propionate,sheep,liver},
  language     = {eng},
  number       = {1},
  pages        = {33--43},
  publisher    = {Elsevier},
  series       = {Livestock Production Science1974-01-01+01:002005-01-01+01:00},
  title        = {Hepatic metabolism of propionate and methylmalonate in growing lambs},
  url          = {http://dx.doi.org/10.1016/S0301-6226(01)00281-0},
  volume       = {74},
  year         = {2002},
}