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Cryogenic probe technology enables multidimensional solid-state NMR of the stratum corneum without isotope labeling

Perrone, Barbara ; Gunnarsson, Maria LU ; Bernin, Diana LU ; Sparr, Emma LU and Topgaard, Daniel LU (2024) In Solid State Nuclear Magnetic Resonance 134.
Abstract

Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables 1H–1H spin diffusion monitored by 2D 1H-13C... (More)

Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables 1H–1H spin diffusion monitored by 2D 1H-13C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids. The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Solid State Nuclear Magnetic Resonance
volume
134
article number
101972
publisher
Elsevier
external identifiers
  • pmid:39357420
  • scopus:85205314390
ISSN
0926-2040
DOI
10.1016/j.ssnmr.2024.101972
language
English
LU publication?
yes
id
3465aca8-f441-46a4-be67-635183531663
date added to LUP
2024-11-27 10:34:18
date last changed
2025-06-26 04:46:12
@article{3465aca8-f441-46a4-be67-635183531663,
  abstract     = {{<p>Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables <sup>1</sup>H–<sup>1</sup>H spin diffusion monitored by 2D <sup>1</sup>H-<sup>13</sup>C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids. The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.</p>}},
  author       = {{Perrone, Barbara and Gunnarsson, Maria and Bernin, Diana and Sparr, Emma and Topgaard, Daniel}},
  issn         = {{0926-2040}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Solid State Nuclear Magnetic Resonance}},
  title        = {{Cryogenic probe technology enables multidimensional solid-state NMR of the stratum corneum without isotope labeling}},
  url          = {{http://dx.doi.org/10.1016/j.ssnmr.2024.101972}},
  doi          = {{10.1016/j.ssnmr.2024.101972}},
  volume       = {{134}},
  year         = {{2024}},
}