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Deoxynivalenol modulated mucin expression and proinflammatory cytokine production, affecting susceptibility to enteroinvasive Escherichia coli infection in intestinal epithelial cells

Wan, Murphy LU ; Co, Vanessa Anna ; Turner, Paul C ; Nagendra, Shah P and El-Nezami, Hani (2025) In Journal of Food Science 90(2). p.1-17
Abstract
Deoxynivalenol (DON) is a common mycotoxin in crops that could induce intestinal inflammation, affecting the susceptibility of intestinal epithelial cells (IECs) to pathogen infection. This study aimed to investigate DON's effects on mucin and cytokine production as part of the local immune system and how it affected intestinal susceptibility to pathogen infection. Caco-2 cells were exposed to DON followed by acute enteroinvasive Escherichia coli (EIEC) infection. An increase in EIEC attachment to DON-exposed cells was observed, probably in part, mediated by secretory MUC5AC mucins and membrane-bound MUC4 and MUC17 mucins. Additionally, DON with EIEC posttreatment led to significant changes in the gene expression of several proinflammatory... (More)
Deoxynivalenol (DON) is a common mycotoxin in crops that could induce intestinal inflammation, affecting the susceptibility of intestinal epithelial cells (IECs) to pathogen infection. This study aimed to investigate DON's effects on mucin and cytokine production as part of the local immune system and how it affected intestinal susceptibility to pathogen infection. Caco-2 cells were exposed to DON followed by acute enteroinvasive Escherichia coli (EIEC) infection. An increase in EIEC attachment to DON-exposed cells was observed, probably in part, mediated by secretory MUC5AC mucins and membrane-bound MUC4 and MUC17 mucins. Additionally, DON with EIEC posttreatment led to significant changes in the gene expression of several proinflammatory cytokines (IL1α, IL1β, IL6, IL8, TNFα, and MCP-1), which may be in part, mediated by NK-κB and/or MAPK signaling pathways. These data suggested DON may exert immunomodulatory effects on IECs, altering the IEC susceptibility to bacterial infection.

Practical Application
The results suggested that DON might modulate immune responses by affecting mucus and cytokine production, which may affect the susceptibility of intestinal epithelial cells to pathogen infection (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Food Science
volume
90
issue
2
article number
e70079
pages
1 - 17
publisher
Wiley-Blackwell
external identifiers
  • scopus:85219372499
  • pmid:39980277
ISSN
0022-1147
DOI
10.1111/1750-3841.70079
language
English
LU publication?
yes
id
34709660-bf5c-43d5-b9d2-dc7c41c571d4
date added to LUP
2025-03-25 21:41:52
date last changed
2025-04-04 15:06:16
@article{34709660-bf5c-43d5-b9d2-dc7c41c571d4,
  abstract     = {{Deoxynivalenol (DON) is a common mycotoxin in crops that could induce intestinal inflammation, affecting the susceptibility of intestinal epithelial cells (IECs) to pathogen infection. This study aimed to investigate DON's effects on mucin and cytokine production as part of the local immune system and how it affected intestinal susceptibility to pathogen infection. Caco-2 cells were exposed to DON followed by acute enteroinvasive Escherichia coli (EIEC) infection. An increase in EIEC attachment to DON-exposed cells was observed, probably in part, mediated by secretory MUC5AC mucins and membrane-bound MUC4 and MUC17 mucins. Additionally, DON with EIEC posttreatment led to significant changes in the gene expression of several proinflammatory cytokines (IL1α, IL1β, IL6, IL8, TNFα, and MCP-1), which may be in part, mediated by NK-κB and/or MAPK signaling pathways. These data suggested DON may exert immunomodulatory effects on IECs, altering the IEC susceptibility to bacterial infection.<br/><br/>Practical Application<br/>The results suggested that DON might modulate immune responses by affecting mucus and cytokine production, which may affect the susceptibility of intestinal epithelial cells to pathogen infection}},
  author       = {{Wan, Murphy and Co, Vanessa Anna and Turner, Paul C and Nagendra, Shah P and El-Nezami, Hani}},
  issn         = {{0022-1147}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{1--17}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Food Science}},
  title        = {{Deoxynivalenol modulated mucin expression and proinflammatory cytokine production, affecting susceptibility to enteroinvasive Escherichia coli infection in intestinal epithelial cells}},
  url          = {{http://dx.doi.org/10.1111/1750-3841.70079}},
  doi          = {{10.1111/1750-3841.70079}},
  volume       = {{90}},
  year         = {{2025}},
}