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Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation

Nilsson, A. M. K.; Heinrich, D.; Olajos, J. and Andersson-Engels, Stefan LU (1997) In Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 53(11). p.1901-1912
Abstract
In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy, spectra in a previously unexplored wavelength region 0.8-2.3 mu m were recorded from various pig heart tissue samples in vitro: normal myocardium (with and without endo/epicardium), aorta; fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA), revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin, collagen and cholesterol as well as with published data. The results obtained... (More)
In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy, spectra in a previously unexplored wavelength region 0.8-2.3 mu m were recorded from various pig heart tissue samples in vitro: normal myocardium (with and without endo/epicardium), aorta; fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA), revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin, collagen and cholesterol as well as with published data. The results obtained with the NIR spectroscopy technique in terms of its potential to classify different tissue types were compared with those from laser-induced fluorescence (LIF) using 337 nm excitation. LIF and NIR spectroscopy can in combination with PCA be used to discriminate between all previously mentioned tissue groups, apart from fatty versus fibrous tissue (LIF) and aorta versus fibrous tissue (NIR), respectively. The NIR analysis was improved by focusing the PCA to the wavelength segment 2.0-2.3 mu m, resulting in successful spectral characterisation of all cardiovascular tissue groups. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Elastin, Cardiovascular tissue, Author Keywords: NIR spectroscopy, Laser-induced fluorescence, Collagen, Cholesterol
in
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
volume
53
issue
11
pages
1901 - 1912
publisher
Elsevier
external identifiers
  • scopus:0031254607
ISSN
1386-1425
DOI
10.1016/S1386-1425(97)00106-6
language
English
LU publication?
yes
id
1f037b0a-6611-4e8b-8da9-1f85a13b22bc (old id 35063)
date added to LUP
2007-07-23 14:49:11
date last changed
2017-10-01 04:37:17
@article{1f037b0a-6611-4e8b-8da9-1f85a13b22bc,
  abstract     = {In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy, spectra in a previously unexplored wavelength region 0.8-2.3 mu m were recorded from various pig heart tissue samples in vitro: normal myocardium (with and without endo/epicardium), aorta; fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA), revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin, collagen and cholesterol as well as with published data. The results obtained with the NIR spectroscopy technique in terms of its potential to classify different tissue types were compared with those from laser-induced fluorescence (LIF) using 337 nm excitation. LIF and NIR spectroscopy can in combination with PCA be used to discriminate between all previously mentioned tissue groups, apart from fatty versus fibrous tissue (LIF) and aorta versus fibrous tissue (NIR), respectively. The NIR analysis was improved by focusing the PCA to the wavelength segment 2.0-2.3 mu m, resulting in successful spectral characterisation of all cardiovascular tissue groups.},
  author       = {Nilsson, A. M. K. and Heinrich, D. and Olajos, J. and Andersson-Engels, Stefan},
  issn         = {1386-1425},
  keyword      = {Elastin,Cardiovascular tissue,Author Keywords: NIR spectroscopy,Laser-induced fluorescence,Collagen,Cholesterol},
  language     = {eng},
  number       = {11},
  pages        = {1901--1912},
  publisher    = {Elsevier},
  series       = {Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy},
  title        = {Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation},
  url          = {http://dx.doi.org/10.1016/S1386-1425(97)00106-6},
  volume       = {53},
  year         = {1997},
}