Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography
Dahlin, Lars B. LU
; Rix, Kristian R.
LU
; Dahl, Vedrana A.
; Dahl, Anders B.
; Jensen, Janus N.
; Cloetens, Peter
; Pacureanu, Alexandra
; Mohseni, Simin
; Thomsen, Niels O.B.
LU
and Bech, Martin
LU
(2020)
In Scientific Reports
10(1).
- Abstract
A deeper knowledge of the architecture of the peripheral nerve with three-dimensional (3D) imaging of the nerve tissue at the sub-cellular scale may contribute to unravel the pathophysiology of neuropathy. Here we demonstrate the feasibility of X-ray phase contrast holographic nanotomography to enable 3D imaging of nerves at high resolution, while covering a relatively large tissue volume. We show various subcomponents of human peripheral nerves in biopsies from patients with type 1 and 2 diabetes and in a healthy subject. Together with well-organized, parallel myelinated nerve fibres we show regenerative clusters with twisted nerve fibres, a sprouted axon from a node of Ranvier and other specific details. A novel 3D construction (with... (More)
A deeper knowledge of the architecture of the peripheral nerve with three-dimensional (3D) imaging of the nerve tissue at the sub-cellular scale may contribute to unravel the pathophysiology of neuropathy. Here we demonstrate the feasibility of X-ray phase contrast holographic nanotomography to enable 3D imaging of nerves at high resolution, while covering a relatively large tissue volume. We show various subcomponents of human peripheral nerves in biopsies from patients with type 1 and 2 diabetes and in a healthy subject. Together with well-organized, parallel myelinated nerve fibres we show regenerative clusters with twisted nerve fibres, a sprouted axon from a node of Ranvier and other specific details. A novel 3D construction (with movie created) of a node of Ranvier with end segment of a degenerated axon and sprout of a regenerated one is captured. Many of these architectural elements are not described in the literature. Thus, X-ray phase contrast holographic nanotomography enables identifying specific morphological structures in 3D in peripheral nerve biopsies from a healthy subject and from patients with type 1 and 2 diabetes.
(Less)
- author
- Dahlin, Lars B.
LU
; Rix, Kristian R.
LU
; Dahl, Vedrana A.
; Dahl, Anders B.
; Jensen, Janus N.
; Cloetens, Peter
; Pacureanu, Alexandra
; Mohseni, Simin
; Thomsen, Niels O.B.
LU
and Bech, Martin
LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- article number
- 7592
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85084206899
- pmid:32371896
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-020-64430-5
- language
- English
- LU publication?
- yes
- id
- 3551ee34-3746-4736-9028-6c74d9999485
- date added to LUP
- 2020-05-27 14:36:27
- date last changed
- 2024-08-07 19:13:59
@article{3551ee34-3746-4736-9028-6c74d9999485,
abstract = {{<p>A deeper knowledge of the architecture of the peripheral nerve with three-dimensional (3D) imaging of the nerve tissue at the sub-cellular scale may contribute to unravel the pathophysiology of neuropathy. Here we demonstrate the feasibility of X-ray phase contrast holographic nanotomography to enable 3D imaging of nerves at high resolution, while covering a relatively large tissue volume. We show various subcomponents of human peripheral nerves in biopsies from patients with type 1 and 2 diabetes and in a healthy subject. Together with well-organized, parallel myelinated nerve fibres we show regenerative clusters with twisted nerve fibres, a sprouted axon from a node of Ranvier and other specific details. A novel 3D construction (with movie created) of a node of Ranvier with end segment of a degenerated axon and sprout of a regenerated one is captured. Many of these architectural elements are not described in the literature. Thus, X-ray phase contrast holographic nanotomography enables identifying specific morphological structures in 3D in peripheral nerve biopsies from a healthy subject and from patients with type 1 and 2 diabetes.</p>}},
author = {{Dahlin, Lars B. and Rix, Kristian R. and Dahl, Vedrana A. and Dahl, Anders B. and Jensen, Janus N. and Cloetens, Peter and Pacureanu, Alexandra and Mohseni, Simin and Thomsen, Niels O.B. and Bech, Martin}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography}},
url = {{http://dx.doi.org/10.1038/s41598-020-64430-5}},
doi = {{10.1038/s41598-020-64430-5}},
volume = {{10}},
year = {{2020}},
}