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The Protein Corona Leads to Deformation of Spherical Micelles

Cao, Cheng ; Zhang, Lin ; Kent, Ben ; Wong, Sandy ; Garvey, Christopher J. LU orcid and Stenzel, Martina H. (2021) In Angewandte Chemie - International Edition 60(18). p.10342-10349
Abstract

The formation of a non-specific protein corona around nanoparticles (NPs) has been identified as one of the culprits for failed nanomedicine. The amount and type of adsorbed protein from the blood plasma are known to determine the fate of NPs and the accessibility of targeting ligands. Herein, we show that the adsorbed protein may not only enlarge the NPs and change their surface properties but also, in the case of soft NPs such as polymer micelles, lead to deformation. Poly(1-O-methacryloyl -β-D-fructopyranose)-b-poly(methylmethacrylate) (P(1-O-MAFru)-b-PMMA) block co-polymers were self-assembled into NPs with a spherical core–shell morphology as determined by small angle neutron scattering (SANS). Upon incubation with albumin, TEM,... (More)

The formation of a non-specific protein corona around nanoparticles (NPs) has been identified as one of the culprits for failed nanomedicine. The amount and type of adsorbed protein from the blood plasma are known to determine the fate of NPs and the accessibility of targeting ligands. Herein, we show that the adsorbed protein may not only enlarge the NPs and change their surface properties but also, in the case of soft NPs such as polymer micelles, lead to deformation. Poly(1-O-methacryloyl -β-D-fructopyranose)-b-poly(methylmethacrylate) (P(1-O-MAFru)-b-PMMA) block co-polymers were self-assembled into NPs with a spherical core–shell morphology as determined by small angle neutron scattering (SANS). Upon incubation with albumin, TEM, SANS, and small angle X-ray scattering (SAXS) revealed the adsorption of albumin and deformation of the NPs with a spheroid geometry. Removal of the protein led to the reversal of the morphology back to the spherical core–shell structure. Structural studies and cell studies of uptake of the NPs imply that the observed deformation may influence blood circulation time and cell uptake.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
drug delivery, glycopolymers, nanoparticles, protein corona, spherical micelles
in
Angewandte Chemie - International Edition
volume
60
issue
18
pages
8 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:33543582
  • scopus:85103282208
ISSN
1433-7851
DOI
10.1002/anie.202101129
language
English
LU publication?
yes
id
3554883b-9441-4ca2-96a1-5ee98e21cdc8
date added to LUP
2021-04-08 10:39:17
date last changed
2024-06-29 10:35:51
@article{3554883b-9441-4ca2-96a1-5ee98e21cdc8,
  abstract     = {{<p>The formation of a non-specific protein corona around nanoparticles (NPs) has been identified as one of the culprits for failed nanomedicine. The amount and type of adsorbed protein from the blood plasma are known to determine the fate of NPs and the accessibility of targeting ligands. Herein, we show that the adsorbed protein may not only enlarge the NPs and change their surface properties but also, in the case of soft NPs such as polymer micelles, lead to deformation. Poly(1-O-methacryloyl -β-D-fructopyranose)-b-poly(methylmethacrylate) (P(1-O-MAFru)-b-PMMA) block co-polymers were self-assembled into NPs with a spherical core–shell morphology as determined by small angle neutron scattering (SANS). Upon incubation with albumin, TEM, SANS, and small angle X-ray scattering (SAXS) revealed the adsorption of albumin and deformation of the NPs with a spheroid geometry. Removal of the protein led to the reversal of the morphology back to the spherical core–shell structure. Structural studies and cell studies of uptake of the NPs imply that the observed deformation may influence blood circulation time and cell uptake.</p>}},
  author       = {{Cao, Cheng and Zhang, Lin and Kent, Ben and Wong, Sandy and Garvey, Christopher J. and Stenzel, Martina H.}},
  issn         = {{1433-7851}},
  keywords     = {{drug delivery; glycopolymers; nanoparticles; protein corona; spherical micelles}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{18}},
  pages        = {{10342--10349}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Angewandte Chemie - International Edition}},
  title        = {{The Protein Corona Leads to Deformation of Spherical Micelles}},
  url          = {{http://dx.doi.org/10.1002/anie.202101129}},
  doi          = {{10.1002/anie.202101129}},
  volume       = {{60}},
  year         = {{2021}},
}