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Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues

Gialeli, Chrysostomi LU and Gonçalves, Isabel LU orcid (2023) In Methods in molecular biology (Clifton, N.J.) 2619. p.91-98
Abstract

Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope
14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric
14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic
14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the... (More)

Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope
14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric
14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic
14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the glycosaminoglycans (GAGs) and subsequent proteoglycans turnover using accelerator mass spectrometry.

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Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Proteoglycans : Methods and Protocols - Methods and Protocols
series title
Methods in molecular biology (Clifton, N.J.)
volume
2619
pages
91 - 98
external identifiers
  • pmid:36662464
  • scopus:85147045898
ISSN
1940-6029
ISBN
978-1-0716-2946-8
DOI
10.1007/978-1-0716-2946-8_7
language
English
LU publication?
yes
additional info
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
id
35c710ea-3fb0-4eac-9958-9417a5013b69
date added to LUP
2023-01-21 16:09:38
date last changed
2024-04-15 19:49:12
@inbook{35c710ea-3fb0-4eac-9958-9417a5013b69,
  abstract     = {{<p>Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope <br>
 14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric<br>
 14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic <br>
 14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the glycosaminoglycans (GAGs) and subsequent proteoglycans turnover using accelerator mass spectrometry.<br>
 </p>}},
  author       = {{Gialeli, Chrysostomi and Gonçalves, Isabel}},
  booktitle    = {{Proteoglycans : Methods and Protocols}},
  isbn         = {{978-1-0716-2946-8}},
  issn         = {{1940-6029}},
  language     = {{eng}},
  pages        = {{91--98}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-2946-8_7}},
  doi          = {{10.1007/978-1-0716-2946-8_7}},
  volume       = {{2619}},
  year         = {{2023}},
}