Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues
(2023) In Methods in molecular biology (Clifton, N.J.) 2619. p.91-98- Abstract
Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope
14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric
14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic
14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the... (More)Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope
(Less)
14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric
14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic
14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the glycosaminoglycans (GAGs) and subsequent proteoglycans turnover using accelerator mass spectrometry.
- author
- Gialeli, Chrysostomi LU and Gonçalves, Isabel LU
- organization
-
- Cardiovascular Research - Translational Studies (research group)
- LUCC: Lund University Cancer Centre
- EXODIAB: Excellence of Diabetes Research in Sweden
- Cardiovascular Research - Immunity and Atherosclerosis (research group)
- Protein Chemistry, Malmö (research group)
- EpiHealth: Epidemiology for Health
- publishing date
- 2023
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Proteoglycans : Methods and Protocols - Methods and Protocols
- series title
- Methods in molecular biology (Clifton, N.J.)
- volume
- 2619
- pages
- 91 - 98
- external identifiers
-
- pmid:36662464
- scopus:85147045898
- ISSN
- 1940-6029
- ISBN
- 978-1-0716-2946-8
- DOI
- 10.1007/978-1-0716-2946-8_7
- language
- English
- LU publication?
- yes
- additional info
- © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- id
- 35c710ea-3fb0-4eac-9958-9417a5013b69
- date added to LUP
- 2023-01-21 16:09:38
- date last changed
- 2024-04-15 19:49:12
@inbook{35c710ea-3fb0-4eac-9958-9417a5013b69, abstract = {{<p>Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope <br> 14C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric<br> 14C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic <br> 14C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the glycosaminoglycans (GAGs) and subsequent proteoglycans turnover using accelerator mass spectrometry.<br> </p>}}, author = {{Gialeli, Chrysostomi and Gonçalves, Isabel}}, booktitle = {{Proteoglycans : Methods and Protocols}}, isbn = {{978-1-0716-2946-8}}, issn = {{1940-6029}}, language = {{eng}}, pages = {{91--98}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues}}, url = {{http://dx.doi.org/10.1007/978-1-0716-2946-8_7}}, doi = {{10.1007/978-1-0716-2946-8_7}}, volume = {{2619}}, year = {{2023}}, }