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Detección de poliovirus en Aguas residuales de Armenia, Colombia

González, Maria M ; Sarmiento, Luis LU ; Castaño, Jhon Carlos ; Giraldo, Alejandra M ; Salazar, Alexander and Muñoz, Nini J (2006) In Revista de Salud Publica 8 (Suppl 1). p.13-23
Abstract

OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.

METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.

RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them... (More)

OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.

METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.

RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.

CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.

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Please use this url to cite or link to this publication:
author
alternative title
Detecting poliovirus in wastewater from Armenia, Colombia
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colombia, Environmental Microbiology, Poliovirus/classification, Poliovirus Vaccine, Oral, Polyethylene Glycols, RNA, Viral/analysis, Reverse Transcriptase Polymerase Chain Reaction, Sewage/virology, Sodium Chloride, Virology/methods
in
Revista de Salud Publica
volume
8
issue
Suppl 1
pages
11 pages
publisher
Universidad Nacional de Colombia
external identifiers
  • pmid:16925118
  • scopus:33747309124
ISSN
0124-0064
language
Spanish
LU publication?
no
id
3622d8f3-e0b4-4940-b914-71bdf010a55d
alternative location
https://www.scielosp.org/article/rsap/2006.v8suppl1/13-23/es/
date added to LUP
2019-07-08 14:50:53
date last changed
2019-08-14 13:38:44
@article{3622d8f3-e0b4-4940-b914-71bdf010a55d,
  abstract     = {<p>OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.</p><p>METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.</p><p>RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.</p><p>CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.</p>},
  author       = {González, Maria M and Sarmiento, Luis and Castaño, Jhon Carlos and Giraldo, Alejandra M and Salazar, Alexander and Muñoz, Nini J},
  issn         = {0124-0064},
  language     = {spa},
  number       = {Suppl 1},
  pages        = {13--23},
  publisher    = {Universidad Nacional de Colombia},
  series       = {Revista de Salud Publica},
  title        = {Detección de poliovirus en Aguas residuales de Armenia, Colombia},
  url          = {https://www.scielosp.org/article/rsap/2006.v8suppl1/13-23/es/},
  volume       = {8 },
  year         = {2006},
}