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Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system

Andersson, Mats; Holmberg, Hans LU and Adlercreutz, Patrick LU (1998) In Biotechnology and Bioengineering 57(1). p.79-86
Abstract

A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions... (More)

A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions catalyzed by HLADH and hydrogenase. Nonimmobilized cells, permeabilized with cetyl- trimethyl-ammonium bromide, were the most efficient preparation for the regeneration of NADH. The use of this preparation in heptane (10% water) was optimized with respect to the yield obtained in the HLADH-catalyzed reduction of cyclohexanone. Using the optimized conditions, yields of 99% cyclohexanol were obtained.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcaligenes eutrophus, HLADH, Hydrogenase, NADH regeneration, Organic solvent
in
Biotechnology and Bioengineering
volume
57
issue
1
pages
8 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:0032484704
ISSN
0006-3592
DOI
10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3
language
English
LU publication?
yes
id
36251d17-f910-4cc2-9caa-4cc12d267875
date added to LUP
2019-06-20 16:09:51
date last changed
2019-10-15 07:08:22
@article{36251d17-f910-4cc2-9caa-4cc12d267875,
  abstract     = {<p>A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions catalyzed by HLADH and hydrogenase. Nonimmobilized cells, permeabilized with cetyl- trimethyl-ammonium bromide, were the most efficient preparation for the regeneration of NADH. The use of this preparation in heptane (10% water) was optimized with respect to the yield obtained in the HLADH-catalyzed reduction of cyclohexanone. Using the optimized conditions, yields of 99% cyclohexanol were obtained.</p>},
  author       = {Andersson, Mats and Holmberg, Hans and Adlercreutz, Patrick},
  issn         = {0006-3592},
  keyword      = {Alcaligenes eutrophus,HLADH,Hydrogenase,NADH regeneration,Organic solvent},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {79--86},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3},
  volume       = {57},
  year         = {1998},
}