Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system
Andersson, Mats ; Holmberg, Hans LU and Adlercreutz, Patrick LU
(1998)
In Biotechnology and Bioengineering
57(1).
p.79-86
- Abstract
A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions... (More)
A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions catalyzed by HLADH and hydrogenase. Nonimmobilized cells, permeabilized with cetyl- trimethyl-ammonium bromide, were the most efficient preparation for the regeneration of NADH. The use of this preparation in heptane (10% water) was optimized with respect to the yield obtained in the HLADH-catalyzed reduction of cyclohexanone. Using the optimized conditions, yields of 99% cyclohexanol were obtained.
(Less)
- author
- Andersson, Mats
; Holmberg, Hans
LU
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 1998-01-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alcaligenes eutrophus, HLADH, Hydrogenase, NADH regeneration, Organic solvent
- in
- Biotechnology and Bioengineering
- volume
- 57
- issue
- 1
- pages
- 8 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:10099181
- scopus:0032484704
- ISSN
- 0006-3592
- DOI
- 10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3
- language
- English
- LU publication?
- yes
- id
- 36251d17-f910-4cc2-9caa-4cc12d267875
- date added to LUP
- 2019-06-20 16:09:51
- date last changed
- 2024-01-30 23:34:02
@article{36251d17-f910-4cc2-9caa-4cc12d267875,
abstract = {{<p>A soluble NAD-dependent hydrogenase contained in Alcaligenes eutrophus was evaluated as a coenzyme regenerating catalyst in an organic-aqueous two- phase (predominantly organic) system. The horse-liver alcohol-dehydrogenase (HLADH) catalyzed reduction of cyclohexanone to cyclohexanol was used as a model reaction. The impact of different solvents (selected to span a large variety of principal properties) on the stability and activity of the HLADH, using substrate-driven regeneration, was studied. Solvents suitable for the HLADH were then selected for an evaluation of the hydrogenase-driven coenzyme regeneration. Hydrophobic solvents such as heptane, toluene; and 1,1,1- trichloroethane were found to be suitable for the coupled reactions catalyzed by HLADH and hydrogenase. Nonimmobilized cells, permeabilized with cetyl- trimethyl-ammonium bromide, were the most efficient preparation for the regeneration of NADH. The use of this preparation in heptane (10% water) was optimized with respect to the yield obtained in the HLADH-catalyzed reduction of cyclohexanone. Using the optimized conditions, yields of 99% cyclohexanol were obtained.</p>}},
author = {{Andersson, Mats and Holmberg, Hans and Adlercreutz, Patrick}},
issn = {{0006-3592}},
keywords = {{Alcaligenes eutrophus; HLADH; Hydrogenase; NADH regeneration; Organic solvent}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{79--86}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Evaluation of Alcaligenes eutrophus cells as an NADH regenerating catalyst in organic-aqueous two-phase system}},
url = {{http://dx.doi.org/10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3}},
doi = {{10.1002/(SICI)1097-0290(19980105)57:1<79::AID-BIT10>3.0.CO;2-3}},
volume = {{57}},
year = {{1998}},
}