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Regulation of bone sialoprotein mRNA by steroid hormones.

Oldberg, Åke LU ; Jirskog-Hed, B; Axelsson, Sara LU and Heinegård, Dick LU (1989) In Journal of Cell Biology 109(6 Pt 1). p.3183-3186
Abstract
In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Messenger: genetics, Messenger: drug effects, RNA, Genes: drug effects, Gene Expression Regulation: drug effects, Dexamethasone: pharmacology, Calcitriol: pharmacology, Bone and Bones: drug effects, Bone and Bones: metabolism, Sialoglycoproteins: genetics, Transcription, Genetic: drug effects
in
Journal of Cell Biology
volume
109
issue
6 Pt 1
pages
3183 - 3186
publisher
Rockefeller University Press
external identifiers
  • pmid:2592421
  • scopus:0024850620
ISSN
0021-9525
language
English
LU publication?
yes
id
c180189c-47e0-450a-9edf-f51b971f2acc (old id 3632721)
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/
date added to LUP
2013-04-10 15:55:21
date last changed
2017-08-06 03:33:18
@article{c180189c-47e0-450a-9edf-f51b971f2acc,
  abstract     = {In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.},
  author       = {Oldberg, Åke and Jirskog-Hed, B and Axelsson, Sara and Heinegård, Dick},
  issn         = {0021-9525},
  keyword      = {Messenger: genetics,Messenger: drug effects,RNA,Genes: drug effects,Gene Expression Regulation: drug effects,Dexamethasone: pharmacology,Calcitriol: pharmacology,Bone and Bones: drug effects,Bone and Bones: metabolism,Sialoglycoproteins: genetics,Transcription,Genetic: drug effects},
  language     = {eng},
  number       = {6 Pt 1},
  pages        = {3183--3186},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Cell Biology},
  title        = {Regulation of bone sialoprotein mRNA by steroid hormones.},
  volume       = {109},
  year         = {1989},
}