Regulation of bone sialoprotein mRNA by steroid hormones.
(1989) In Journal of Cell Biology 109(6 Pt 1). p.3183-3186- Abstract
- In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3632721
- author
- Oldberg, Åke LU ; Jirskog-Hed, B ; Axelsson, Sara LU and Heinegård, Dick LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Messenger: genetics, Messenger: drug effects, RNA, Genes: drug effects, Gene Expression Regulation: drug effects, Dexamethasone: pharmacology, Calcitriol: pharmacology, Bone and Bones: drug effects, Bone and Bones: metabolism, Sialoglycoproteins: genetics, Transcription, Genetic: drug effects
- in
- Journal of Cell Biology
- volume
- 109
- issue
- 6 Pt 1
- pages
- 3183 - 3186
- publisher
- Rockefeller University Press
- external identifiers
-
- pmid:2592421
- scopus:0024850620
- ISSN
- 0021-9525
- language
- English
- LU publication?
- yes
- id
- c180189c-47e0-450a-9edf-f51b971f2acc (old id 3632721)
- alternative location
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/
- date added to LUP
- 2016-04-01 11:44:17
- date last changed
- 2021-09-26 05:09:30
@article{c180189c-47e0-450a-9edf-f51b971f2acc, abstract = {{In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.}}, author = {{Oldberg, Åke and Jirskog-Hed, B and Axelsson, Sara and Heinegård, Dick}}, issn = {{0021-9525}}, keywords = {{Messenger: genetics; Messenger: drug effects; RNA; Genes: drug effects; Gene Expression Regulation: drug effects; Dexamethasone: pharmacology; Calcitriol: pharmacology; Bone and Bones: drug effects; Bone and Bones: metabolism; Sialoglycoproteins: genetics; Transcription; Genetic: drug effects}}, language = {{eng}}, number = {{6 Pt 1}}, pages = {{3183--3186}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Cell Biology}}, title = {{Regulation of bone sialoprotein mRNA by steroid hormones.}}, url = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/}}, volume = {{109}}, year = {{1989}}, }