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Acellular human lung scaffolds to model lung disease and tissue regeneration

Gilpin, Sarah E. and Wagner, Darcy E. LU (2018) In European Respiratory Review 27(148).
Abstract

Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed using both perfusion and immersion based techniques. Immersion based techniques allow laboratories without access to intact lobes the ability to generate acellular human lung scaffolds. Acellular human lung scaffolds can be further processed into small segments, thin slices or extracellular matrix extracts, to study cell behaviour such as viability, proliferation, migration and differentiation. Recent studies have offered important proof of... (More)

Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed using both perfusion and immersion based techniques. Immersion based techniques allow laboratories without access to intact lobes the ability to generate acellular human lung scaffolds. Acellular human lung scaffolds can be further processed into small segments, thin slices or extracellular matrix extracts, to study cell behaviour such as viability, proliferation, migration and differentiation. Recent studies have offered important proof of concept of generating sufficient primary endothelial and lung epithelial cells to recellularise whole lobes that can be maintained for several days ex vivo in a bioreactor to study regeneration. In parallel, acellular human lung scaffolds have been increasingly used for studying cell-extracellular environment interactions. These studies have helped provide new insights into the role of the matrix and the extracellular environment in chronic human lung diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. Acellular human lung scaffolds are a versatile new tool for studying human lung repair and regeneration ex vivo.

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author
organization
publishing date
type
Contribution to journal
publication status
published
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in
European Respiratory Review
volume
27
issue
148
publisher
European Respiratory Society
external identifiers
  • scopus:85048345019
ISSN
0905-9180
DOI
10.1183/16000617.0021-2018
language
English
LU publication?
yes
id
363a8e6c-449c-4038-adc5-2f98516a0dd1
date added to LUP
2018-06-25 14:18:01
date last changed
2018-07-29 04:35:04
@article{363a8e6c-449c-4038-adc5-2f98516a0dd1,
  abstract     = {<p>Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed using both perfusion and immersion based techniques. Immersion based techniques allow laboratories without access to intact lobes the ability to generate acellular human lung scaffolds. Acellular human lung scaffolds can be further processed into small segments, thin slices or extracellular matrix extracts, to study cell behaviour such as viability, proliferation, migration and differentiation. Recent studies have offered important proof of concept of generating sufficient primary endothelial and lung epithelial cells to recellularise whole lobes that can be maintained for several days ex vivo in a bioreactor to study regeneration. In parallel, acellular human lung scaffolds have been increasingly used for studying cell-extracellular environment interactions. These studies have helped provide new insights into the role of the matrix and the extracellular environment in chronic human lung diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. Acellular human lung scaffolds are a versatile new tool for studying human lung repair and regeneration ex vivo.</p>},
  articleno    = {180021},
  author       = {Gilpin, Sarah E. and Wagner, Darcy E.},
  issn         = {0905-9180},
  language     = {eng},
  month        = {06},
  number       = {148},
  publisher    = {European Respiratory Society},
  series       = {European Respiratory Review},
  title        = {Acellular human lung scaffolds to model lung disease and tissue regeneration},
  url          = {http://dx.doi.org/10.1183/16000617.0021-2018},
  volume       = {27},
  year         = {2018},
}