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Hyperglycemia and hypercapnia suppress BDNF gene expression in vulnerable regions after transient forebrain ischemia in the rat

Uchino, H. LU ; Lindvall, O. LU ; Siesjö, B. K. LU and Kokaia, Zaal LU (1997) In Journal of Cerebral Blood Flow and Metabolism 17(12). p.1303-1308
Abstract

Preischemic hyperglycemia or superimposed hypercapnia exaggerates brain damage caused by transient forebrain ischemia. Because high regional levels of brain-derived neurotrophic factor (BDNF) protein correlate with resistance to ischemic damage, we studied the expression of BDNF mRNA using in sire hybridization in rats subjected to 10 minutes of forebrain ischemia under normoglycemic, hyperglycemic, or hypercapnic conditions. Compared with normoglycemic animals, the increase of BDNF mRNA in dentate granule cells was attenuated and that in CA3 pyramidal neurons completely prevented in hyperglycemic rats. No ischemia-induced increases of BDNF mRNA levels in the hippocampal formation were detected in hypercapnic animals. Hyperglycemic and... (More)

Preischemic hyperglycemia or superimposed hypercapnia exaggerates brain damage caused by transient forebrain ischemia. Because high regional levels of brain-derived neurotrophic factor (BDNF) protein correlate with resistance to ischemic damage, we studied the expression of BDNF mRNA using in sire hybridization in rats subjected to 10 minutes of forebrain ischemia under normoglycemic, hyperglycemic, or hypercapnic conditions. Compared with normoglycemic animals, the increase of BDNF mRNA in dentate granule cells was attenuated and that in CA3 pyramidal neurons completely prevented in hyperglycemic rats. No ischemia-induced increases of BDNF mRNA levels in the hippocampal formation were detected in hypercapnic animals. Hyperglycemic and hypercapnic rats showed transiently decreased expression of BDNF mRNA levels in the cingulate cortex, which was not observed in normoglycemic animals. The results suggest that suppression of the BDNF gene might contribute to the increased vulnerability of the CA3 region and cingulate cortex in hyperglycemic and hypercapnic animals.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BDNF, Cerebral ischemia, Gene expression, Hypercapnia, Hyperglycemia, In situ hybridization
in
Journal of Cerebral Blood Flow and Metabolism
volume
17
issue
12
pages
1303 - 1308
publisher
Nature Publishing Group
external identifiers
  • pmid:9397029
  • scopus:0030670532
ISSN
0271-678X
DOI
10.1097/00004647-199712000-00005
language
English
LU publication?
no
id
366c21df-6c0f-498d-a23b-92e7046c71ac
date added to LUP
2019-09-03 17:05:48
date last changed
2020-01-13 02:19:41
@article{366c21df-6c0f-498d-a23b-92e7046c71ac,
  abstract     = {<p>Preischemic hyperglycemia or superimposed hypercapnia exaggerates brain damage caused by transient forebrain ischemia. Because high regional levels of brain-derived neurotrophic factor (BDNF) protein correlate with resistance to ischemic damage, we studied the expression of BDNF mRNA using in sire hybridization in rats subjected to 10 minutes of forebrain ischemia under normoglycemic, hyperglycemic, or hypercapnic conditions. Compared with normoglycemic animals, the increase of BDNF mRNA in dentate granule cells was attenuated and that in CA3 pyramidal neurons completely prevented in hyperglycemic rats. No ischemia-induced increases of BDNF mRNA levels in the hippocampal formation were detected in hypercapnic animals. Hyperglycemic and hypercapnic rats showed transiently decreased expression of BDNF mRNA levels in the cingulate cortex, which was not observed in normoglycemic animals. The results suggest that suppression of the BDNF gene might contribute to the increased vulnerability of the CA3 region and cingulate cortex in hyperglycemic and hypercapnic animals.</p>},
  author       = {Uchino, H. and Lindvall, O. and Siesjö, B. K. and Kokaia, Zaal},
  issn         = {0271-678X},
  language     = {eng},
  number       = {12},
  pages        = {1303--1308},
  publisher    = {Nature Publishing Group},
  series       = {Journal of Cerebral Blood Flow and Metabolism},
  title        = {Hyperglycemia and hypercapnia suppress BDNF gene expression in vulnerable regions after transient forebrain ischemia in the rat},
  url          = {http://dx.doi.org/10.1097/00004647-199712000-00005},
  doi          = {10.1097/00004647-199712000-00005},
  volume       = {17},
  year         = {1997},
}