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Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia

Shamloo, Mehrdad ; Soriano, Liza ; von Schack, David ; Rickhag, Mattias ; Chin, Daniel J. ; Gonzalez-Zulueta, Mirella ; Gidö, Gunilla ; Urfer, Roman ; Wieloch, Tadeusz LU and Nikolich, Karoly (2006) In European Journal of Neuroscience 24(10). p.2705-2720
Abstract
Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly... (More)
Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of similar to 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rat, NXF, hypoxia-inducing factor 1 alpha, brain, hypoxia, stroke
in
European Journal of Neuroscience
volume
24
issue
10
pages
2705 - 2720
publisher
Wiley-Blackwell
external identifiers
  • wos:000242221700003
  • scopus:33751360974
  • pmid:17156197
ISSN
1460-9568
DOI
10.1111/j.1460-9568.2006.05172.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000)
id
54a1778c-c842-44e0-98f8-fdbd2fc76d88 (old id 376618)
date added to LUP
2016-04-01 11:43:09
date last changed
2021-10-06 04:41:43
@article{54a1778c-c842-44e0-98f8-fdbd2fc76d88,
  abstract     = {Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of similar to 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury.},
  author       = {Shamloo, Mehrdad and Soriano, Liza and von Schack, David and Rickhag, Mattias and Chin, Daniel J. and Gonzalez-Zulueta, Mirella and Gidö, Gunilla and Urfer, Roman and Wieloch, Tadeusz and Nikolich, Karoly},
  issn         = {1460-9568},
  language     = {eng},
  number       = {10},
  pages        = {2705--2720},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia},
  url          = {http://dx.doi.org/10.1111/j.1460-9568.2006.05172.x},
  doi          = {10.1111/j.1460-9568.2006.05172.x},
  volume       = {24},
  year         = {2006},
}